Antibacterial Activity Analysis of Hydroxyapatite Based Materials with Fluorine and Silver.
This investigation aims to evaluate the antibacterial activity of nanostructured hydroxyapatite based materials doped with silver and fluorine, to be used as a biomaterial with antibacterial activity. Four different formulations were prepared by combustion method: hydroxyapatite, hydroxyapatite-fluo...
| Publicado en: | Revista Mexicana de Ingeniería Biomédica Vol. 42; no. 2; pp. 49 - 58 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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Sociedad Mexicana de Ingenieria Biomedica, A.C.
May-Aug2021
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=150686548&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 150686548 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 01889532 7L3 jtl: Revista Mexicana de Ingeniería Biomédica issn: 01889532 maglogo: N pubinfo: dt: May-Aug2021 vid: 42 iid: 2 pid: 20850 pub: Sociedad Mexicana de Ingenieria Biomedica, A.C. artinfo: ui: 150686548 10.17488/RMIB.42.2.4 ppf: 49 ppct: 9 formats: fmt: @attributes: type: P size: 1.9MB tig: atl: Antibacterial Activity Analysis of Hydroxyapatite Based Materials with Fluorine and Silver. aug: au: González-Torres, V. Hernández-Guevara, E. Castillo-Martínez, N. A. Rosales-Aguilar, M. Díaz-Trujillo, G. C. affil: Facultad de Ciencias de la Salud de la Universidad Autónoma de Baja California Facultad de Ciencias Químicas e Ingeniería de la Universidad Autónoma de Baja California Facultad de Medicina y Psicología de la Universidad Autónoma de Baja California su: Antibacterial agents Hydroxyapatite Fluorine Silver Nanostructured materials sug: subj: Antibacterial agents Hydroxyapatite Fluorine Silver Nanostructured materials keyword: antibacterial hydroxyapatite silver hidroxiapatita plata ab: This investigation aims to evaluate the antibacterial activity of nanostructured hydroxyapatite based materials doped with silver and fluorine, to be used as a biomaterial with antibacterial activity. Four different formulations were prepared by combustion method: hydroxyapatite, hydroxyapatite-fluorine, hydroxyapatite-silver-fluorine and hydroxyapatite-silver, with 2% of the doping agents. X-ray diffraction technique was used to determine the mineralogy, identifying the presence of Ca(PO)OH, CaPO, AgPO, AgCa(PO) Ca(PO)F and CaF phases for the studied samples. Scanning electron microscopy was used to study the morphological structure and it showed homogeneous crystallization of the hydroxyapatite and the inclusion of dopant agents. The antibacterial activity was determined using a modified inhibition test zone to observe if the bacteria (E. faecalis) was susceptible to the antimicrobial agent by the appearance of the zone of inhibition on the agar plate. Both the hydroxyapatite-silver and the hydroxyapatite-silver-fluorine materials generated an inhibition zone. It was possible to determine the minimum inhibitory concentration needed to kill most viable organisms after 48 hours of incubation using the broth microdilution method, resulting in 75 µg/ml and 200 µg/ml for the hydroxyapatite-silver and the hydroxyapatite-silver-fluorine formulation, respectively. These materials could be used for the development of new biomaterials that can be used in dental applications. El objetivo de esta investigación es analizar la actividad antibacteriana de materiales nanoestructurados a base de hidroxiapatita con iones de flúor y plata que le confieran características particulares para que pueda ser utilizado como un biomaterial con actividad antimicrobiana. Se realizaron cuatro formulaciones distintas: hidroxiapatita, hidroxiapatita-flúor, hidroxiapatita-plata-flúor e hidroxiapatita-plata con un 2% de los agentes dopantes. La síntesis del material se realizó a través del método de combustión. La caracterización mineral se realizó a través de difracción de rayos X identificando las siguientes fases en las diversas formulaciones: Ca(PO)OH, CaPO, AgPO, AgCa(PO) Ca(PO)F and CaF. La estructura morfológica se analizó a través de microscopía electrónica de barrido que muestra la formación de estructuras compactas, presencia de cristales y la incrustación de flúor y plata. Se analizó la actividad antimicrobiana utilizando una prueba modificada para la observación del halo de inhibición, encontrándose solamente que los materiales que contenían plata-flúor y plata generaron dicho halo de inhibición. Por otra parte, usando la prueba de microdilución en pozo se encontró que la concentración mínima inhibitoria para el material de HA-Ag fue de 75 µg/ml y para el material de HA-Ag-F fue de 200 µg/ml, después de 48 horas de incubación utilizando E. faecalis. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Mexicana de Ingeniería Biomédica is the property of Sociedad Mexicana de Ingenieria Biomedica, A.C. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Mexicana de Ingeniería Biomédica holder: Sociedad Mexicana de Ingenieria Biomedica, A.C. dt: @attributes: year: 2021 holdings: @attributes: islocal: N |
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