Marker Genes Change of Synovial Fibroblasts in Rheumatoid Arthritis Patients.
Background. Rheumatoid arthritis (RA) is a chronic condition that manifests as inflammation of synovial joints, leading to joint destruction and deformity. Methods. We identified single-cell RNA-seq data of synovial fibroblasts from RA and osteoarthritis (OA) patients in GSE109449 dataset. RA- and O...
| Published in: | BioMed Research International pp. 1 - 18 |
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| Main Authors: | , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
6/7/2021
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=150749110&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 150749110 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 6/7/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 150749110 150749110 150749110 10.1155/2021/5544264 150749110 ppf: 1 ppct: 17 formats: fmt: @attributes: type: P tig: atl: Marker Genes Change of Synovial Fibroblasts in Rheumatoid Arthritis Patients. aug: au: Liao, Lifen Liang, Ke Lan, Lan Wang, Jinheng Guo, Jun affil: Department of Laboratory, Affiliated Hospital of Guilin Medical University, Xiufeng District, Guilin, 541001 Guangxi, China sug: subj: Genetic Markers Metabolism Synovial Membrane Metabolism Fibroblasts Metabolism Arthritis, Rheumatoid Familial and Genetic Arthritis, Rheumatoid Physiopathology Osteoarthritis Familial and Genetic Osteoarthritis Physiopathology RNA Metabolism Gene Expression Sequence Analysis Human Descriptive Statistics ROC Curve Arthritis, Rheumatoid Osteoarthritis Immunology Flow Cytometry Polymerase Chain Reaction Blotting, Western Neutrophils Immunity Chemokines Metabolism Inflammation Mediators Metabolism Matrix Metalloproteinases Metabolism Biological Markers Metabolism Arthritis, Rheumatoid Diagnosis B Lymphocytes Metabolism Antigens, Surface Metabolism Extracellular Space Metabolism Receptors, Cell Surface Metabolism ab: Background. Rheumatoid arthritis (RA) is a chronic condition that manifests as inflammation of synovial joints, leading to joint destruction and deformity. Methods. We identified single-cell RNA-seq data of synovial fibroblasts from RA and osteoarthritis (OA) patients in GSE109449 dataset. RA- and OA-specific cellular subpopulations were identified, and enrichment analysis was performed. Further, key genes for RA and OA were obtained by combined analysis with differentially expressed genes (DEGs) between RA and OA in GSE56409 dataset. The diagnostic role of key genes for RA was predicted using receiver operating characteristic (ROC) curve. Finally, we identified differences in immune cell infiltration between RA and OA patients, and utilized flow cytometry, qRT-PCR, and Western blot were used to examine the immune cell and key genes in RA patients. Results. The cluster 0 matched OA and cluster 3 matched RA and significantly enriched for neutrophil-mediated immunity and ECM receptor interaction, respectively. We identified 478 DEGs. In the top 20 degrees of connection in the PPI network, the key genes for RA were obtained by comparing with the gene markers of cluster 0 and cluster 3, respectively. ROC curve showed that CCL2 and MMP13 might be diagnostic markers for RA. We found aberrant levels of CD8+T, neutrophil, and B cells in RA fibroblasts, which were validated in clinical samples. Importantly, we also validated the differential expression of key genes between RA and OA. Conclusion. High expression of CCL2 and MMP13 in RA may be a diagnostic and therapeutic target. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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