Antitumor Activity of Ethanolic Extract from Thymbra Spicata L. aerial Parts: Effects on Cell Viability and Proliferation, Apoptosis Induction, STAT3, and NF-kB Signaling.

Thyme-like plants including Thymbra spicata L. are widely used as food and folk medicinal remedies in the Mediterranean area. This study aimed to explore the in vitro antitumor potential of polyphenol-enriched extracts from aerial parts of T. spicata. The ethanolic extract significantly inhibited pr...

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Published in:Nutrition & Cancer Vol. 73; no. 7; pp. 1193 - 1207
Main Authors: Khalil, Mohamad, Khalifeh, Hala, Baldini, Francesca, Serale, Nadia, Parodi, Alessia, Voci, Adriana, Vergani, Laura, Daher, Ahmad
Format: pictorial research tables/charts Journal Article
Published: Taylor & Francis Ltd 2021
Online Access:View this record in EBSCOhost
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      dt: 2021
      vid: 73
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      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        10.1080/01635581.2020.1792517
        150797816
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        atl: Antitumor Activity of Ethanolic Extract from Thymbra Spicata L. aerial Parts: Effects on Cell Viability and Proliferation, Apoptosis Induction, STAT3, and NF-kB Signaling.
      aug:
        au:
          Khalil, Mohamad
          Khalifeh, Hala
          Baldini, Francesca
          Serale, Nadia
          Parodi, Alessia
          Voci, Adriana
          Vergani, Laura
          Daher, Ahmad
        affil: Laboratory Rammal Rammal (ATAC), Faculty of Sciences, Lebanese University, Beirut, Lebanon
      sug:
        subj:
          Ethanol
          Plant Extracts Pharmacodynamics
          Antineoplastic Agents Pharmacodynamics
          Polyphenols
          Plant Components, Aerial
          Cell Viability
          Cell Proliferation
          Apoptosis
          Carrier Proteins
          Human
          In Vitro Studies
          Molecular Structure
          NF-kappa B
          Signal Transduction
          Cell Line, Tumor
          Breast Neoplasms
          Descriptive Statistics
          Wound Healing
          Cytotoxins
          Blotting, Western
      ab: Thyme-like plants including Thymbra spicata L. are widely used as food and folk medicinal remedies in the Mediterranean area. This study aimed to explore the in vitro antitumor potential of polyphenol-enriched extracts from aerial parts of T. spicata. The ethanolic extract significantly inhibited proliferation of different human tumor cell lines, without significant effects on non-neoplastic cells. A deeper investigation of the molecular mechanism sustaining the in vitro antitumor activity of the extract was carried on the human breast cancer cells MCF-7 in comparison with the normal breast cells MCF-10A. The effects on MCF-7 cells were associated with the following: (i) production of reactive oxygen species (ROS) and release of nitric oxide; (ii) apoptosis induction; and (iii) reduction in STAT3 and NF-kB phosphorylation. The ethanolic extract from T. spicata leaves might represent a novel therapeutic tool in combination with conventional chemotherapy to reduce the adverse side effects and drug resistance.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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