Operational characteristics of an antibody detecting point of care test for Taenia solium infections in a community and hospital setting.

Background: Diagnostic test evaluation includes measures of performance and assessment of operational characteristics. The latter focuses on end-user understanding of instructions to perform the test, ease of use, test turnaround time and ease of result interpretation. This study aimed to assess use...

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Publicado en:BMC Infectious Diseases Vol. 21; no. 1; pp. 1 - 10
Autores principales: Mubanga, Chishimba, Mwape, Kabemba E., Phiri, Isaac K., Trevisan, Chiara, Kabululu, Mwemezi, Zulu, Gideon, Van Damme, Inge, Schmidt, Veronika, Dorny, Pierre, Gabriël, Sarah
Formato: Journal Article
Publicado: BioMed Central 6/25/2021
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 6/25/2021
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      pub: BioMed Central
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        10.1186/s12879-021-06320-3
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        atl: Operational characteristics of an antibody detecting point of care test for Taenia solium infections in a community and hospital setting.
      aug:
        au:
          Mubanga, Chishimba
          Mwape, Kabemba E.
          Phiri, Isaac K.
          Trevisan, Chiara
          Kabululu, Mwemezi
          Zulu, Gideon
          Van Damme, Inge
          Schmidt, Veronika
          Dorny, Pierre
          Gabriël, Sarah
        affil: Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia
      sug:
        subj:
          Cestodes
          Taeniasis Diagnosis
          Adult
          Animals
          Female
          Young Adult
          Hospitals
          Middle Age
          Tanzania
          Cysticercosis Diagnosis
          Community Health Services
          Antibodies Blood
          Zambia
          Male
          Checklists
          Scales
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Background: Diagnostic test evaluation includes measures of performance and assessment of operational characteristics. The latter focuses on end-user understanding of instructions to perform the test, ease of use, test turnaround time and ease of result interpretation. This study aimed to assess user comprehension of training for and ease of use of a Taenia solium point of care test (TS POC) evaluated in a community and hospital setting in Zambia and Tanzania, respectively.Methods: The TS POC is a three-step in-house-produced rapid diagnostic test (RDT) for the simultaneous detection of taeniosis (TST) and cysticercosis (TSCC) antibodies. Data collected by administering questionnaires to 29 end-users and from the main evaluation database was analyzed quantitatively.Results: End-users (28/29, 97%) perceived that the training they received for performing the test was sufficient. They performed 4080 tests, of which 80 were invalid. The community-based study and TST tests had higher invalid rates. The overall result interpretation was within the acceptable range of RDTs with an overall disagreement between readers of 3.3%. The Kappa coefficient of agreement was 85 and 82% for TSCC and TST, respectively. There was more disagreement among readers in the community-based study.Conclusion: End-users rated the TS POC kit moderate in terms of ease of use citing long test turnaround time and difficulties in using the blood transfer device. Overall, the operational performance of the TS POC kit and end-users was within the established acceptable performance range.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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