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Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells.

Introduction: Gender-specific phenotypes of the heart were reported with respect to both physiology and pathology. While most differences were associated with the sex hormones, differential expression of genes received special attention, particularly X-Y chromosomes' genes. Methods: Here, we compare...

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Published in:Journal of Cardiovascular & Thoracic Research Vol. 13; no. 2; pp. 146 - 156
Main Authors: Ahmadvand, Shiva, Osia, Ali, Meyfour, Anna, Pahlavan, Sara
Format: pictorial research tables/charts Journal Article
Published: Tabriz University of Medical Sciences 2021
Online Access:View this record in EBSCOhost
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        20085117
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      dt: 2021
      vid: 13
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      pub: Tabriz University of Medical Sciences
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        151253814
        151253814
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        10.34172/jcvtr.2021.32
        151253814
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        atl: Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells.
      aug:
        au:
          Ahmadvand, Shiva
          Osia, Ali
          Meyfour, Anna
          Pahlavan, Sara
        affil: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
      sug:
        subj:
          Sex Factors
          Phenotype Physiology
          Cardiomyopathy, Hypertrophic Pathology
          Myocytes, Cardiac
          Gene Expression
          Cell Differentiation
          Stem Cells
          Sex Hormones
          Gene Expression Profiling
          Chromosomes
          Genes
          Heart Pathology
          Genetic Markers
          In Vitro Studies
          Isoproterenol
          Data Analysis Software
          Male
          Female
          Human
          Stem Cell Research
          Male
          Female
      ab: Introduction: Gender-specific phenotypes of the heart were reported with respect to both physiology and pathology. While most differences were associated with the sex hormones, differential expression of genes received special attention, particularly X-Y chromosomes' genes. Methods: Here, we compared cardiogenesis by gene expression analysis of lineage specific markers and X-Y chromosomes' genes, during in vitro differentiation of XY and XX human embryonic stem cells (hESC), in a hormone-free setup. Results: Downregulation of pluripotency marker (NANOG) and upregulation of cardiac mesoderm and progenitor markers (GATA4, TBX5, NKX2.5, ISL1) was remained temporally similar in differentiating XY and XX hESCs. Isoproterenol treatment of XY and XX hESC-derived cardiomyocytes (hESCCM) induced hypertrophy in a sex-specific manner, with female cardiomyocytes showing response at higher isoproterenol concentration and a later time point of differentiation. Interestingly, KDM5C as an X-linked gene, was markedly upregulated in both hypertrophied male and female cardiomyocytes. Conclusion: Collectively, our results indicated a temporally identical cardiogenesis, but more susceptibility of XY hESC-CM to hypertrophic stimulus in a hormone-free condition.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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