The Level of Mesenchymal-Epithelial Transition Autophosphorylation is Correlated with Esophageal Squamous Cell Carcinoma Migration.
Background: The MET receptor is a critical member of cancer-associated receptor tyrosine kinases and plays an important role in different biological activities, including differentiation, migration, and cell proliferation.Methods: In this study, novel MET inhibitors were introduced and applied on es...
| Publicado en: | Iranian Biomedical Journal Vol. 25; no. 4; pp. 243 - 255 |
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| Autores principales: | , , , |
| Formato: | research Journal Article |
| Publicado: |
Pasteur Institute of Iran
Jul2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=151297185&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 151297185 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1028852X 783E jtl: Iranian Biomedical Journal issn: 1028852X maglogo: N pubinfo: dt: Jul2021 vid: 25 iid: 4 pid: 47405 pub: Pasteur Institute of Iran artinfo: ui: 151297185 151297185 NLM34217156 151297185 10.52547/ibj.25.4.243 NLM34217156 151297185 ppf: 243 ppct: 12 formats: tig: atl: The Level of Mesenchymal-Epithelial Transition Autophosphorylation is Correlated with Esophageal Squamous Cell Carcinoma Migration. aug: au: Taghehchian, Negin Moghbeli, Meysam Mashkani, Baratali Abbaszadegan, Mohammad Reza affil: Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran sug: subj: Esophageal Neoplasms Metabolism Cell Movement Physiology Esophageal Neoplasms Dose-Response Relationship, Drug Antineoplastic Agents Pharmacodynamics Cell Line, Tumor Cell Physiology Physiology Phosphorylation Physiology Human ab: Background: The MET receptor is a critical member of cancer-associated receptor tyrosine kinases and plays an important role in different biological activities, including differentiation, migration, and cell proliferation.Methods: In this study, novel MET inhibitors were introduced and applied on esophageal squamous carcinoma cell line KYSE-30, and the level of proliferation and migration, as well as the activated form of MET receptor protein were assessed in the examined cells. The human KYSE-30 cell line was cultured according to ATCC recommendations. The mRNA level of the MET gene was measured in the examined cell line using the quantitative RT-PCR assay. Cytotoxicity evaluation test was performed at different concentrations of heterocyclic anti-MET compounds (i.e. D1, D2, D5, D6, D7, and D8). Finally, the capability of these compounds in MET receptor inhibition was evaluated using the migration assay and Western blot. All experiments were performed in triplicate and repeated three times with similar results.Results: Cell growth and proliferation were significantly inhibited (p ≤ 0.05) by all the above-mentioned compounds. Moreover, the majority of compounds significantly prevented the cell migration (p ≤ 0.05) and inhibited MET autophosphorylation. Interestingly, the level of phosphorylated MET was significantly correlated with KYSE-30 cell migration.Conclusion: The obtained data introduced and confirmed the biological activities of the mentioned novel compounds in KYSE-30 cells and proposed that the therapeutic inhibition of MET with these compounds may be a powerful approach for inhibiting cancer cell migration and proliferation although some structural optimizations are needed to improve their inhibitory functions. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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