Reusable, Noninvasive, and Sensitive Fluorescence Enhanced ZnO-Nanorod-Based Microarrays for Quantitative Detection of AFP in Human Serum.
The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by t...
| Publicado en: | BioMed Research International pp. 1 - 12 |
|---|---|
| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
7/16/2021
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=151429476&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 151429476 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 7/16/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 151429476 151429476 151429476 10.1155/2021/9916909 151429476 ppf: 1 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Reusable, Noninvasive, and Sensitive Fluorescence Enhanced ZnO-Nanorod-Based Microarrays for Quantitative Detection of AFP in Human Serum. aug: au: Rafique, Saima Kiyani, Farukh Jawaid, Sumbal Nasir, Rubina Ahmad, Mahmoosh Bashir, Shazia Idress, Muhammad Khan, Jan Sher Akram, Rizwan affil: Department of Physics, Air University, PAF Complex, E-9, Islamabad 44000, Pakistan sug: subj: Zinc Oxide Nanostructures alpha Fetoproteins Blood Serum Analysis Protein Array Analysis Methods Fluoroimmunoassay Methods Noninvasive Procedures Human Quantitative Studies Blood Proteins Fluorometry Microscopy, Electron, Scanning Sensitivity and Specificity Tumor Markers, Biological Neoplasms Diagnosis Recovery Protein Array Analysis Economics Cost Benefit Analysis Research, Medical ab: The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by the use of ZnO-nanorods and is investigated as a substrate which enhance the florescent signal to diagnose the hepatocellular carcinoma (HCC) at early stages. The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-nanorods enhance the fluorescent signal 7.2 times as compared to the substrate without ZnO-nanorods. The microarray showed sensitivity of 1504.7 ng ml-1 and limit of detection of 0.1 pg ml-1 in wide dynamic range of 0.05 pg-10 μg ml-1 for alpha fetoprotein (AFP) detection in 10% human serum. This immunoassay was successfully applied for human serum samples to detect tumor marker with good recoveries. The ZnO-nanorod substrate is a simple protein microarray which showed a great promise for developing a low-cost, sensitive, and high-throughput protein assay platform for several applications in both fundamental research and clinical diagnosis. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|