ARHGEF3 Associated with Invasion, Metastasis, and Proliferation in Human Osteosarcoma.
Background. Osteosarcoma is a malignant bone tumor composed of mesenchymal cells producing osteoid and immature bone. This study is aimed at developing novel potential prognostic biomarkers and constructing a miRNA-mRNA network for progression in osteosarcoma. Method. GSE70367 and GSE70414 were obta...
| Publicado en: | BioMed Research International pp. 1 - 13 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
7/26/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=151593284&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 151593284 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 7/26/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 151593284 151593284 151593284 10.1155/2021/3381957 151593284 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: ARHGEF3 Associated with Invasion, Metastasis, and Proliferation in Human Osteosarcoma. aug: au: Gong, Jie Tang, Wei Lv, Bin Zhang, Shushu Fan, Tingjuan Gao, Guangyu Chen, Dong Liu, Yulong affil: Department of Orthopedics, Suzhou Xiangcheng People's Hospital, 1060 Huayuan Road, Suzhou 215004, China sug: subj: Neoplasm Invasiveness Osteosarcoma Prognosis Neoplasm Metastasis Cell Proliferation MicroRNA Tumor Markers, Biological Gene Expression RNA, Messenger Blotting, Western Reverse Transcriptase Polymerase Chain Reaction Survival Analysis Kaplan-Meier Estimator Microarray Analysis Bioinformatics Descriptive Statistics Human ab: Background. Osteosarcoma is a malignant bone tumor composed of mesenchymal cells producing osteoid and immature bone. This study is aimed at developing novel potential prognostic biomarkers and constructing a miRNA-mRNA network for progression in osteosarcoma. Method. GSE70367 and GSE70414 were obtained in the Gene Expression Omnibus (GEO) database. GEO software and the GEO2R calculation method were used to analyze two gene profiles. The coexpression of differentially expressed miRNAs (DEMs) and genes (DEGs) was identified and searched for in the FunRich database for pathway and ontology analysis. Cytoscape was utilized to construct the mRNA-miRNA network. Survival analysis of identified miRNAs and mRNAs was performed by utilizing the Kaplan-Meier Plotter. Besides, expression levels of DEMs and target mRNAs were verified by performing quantitative real-time PCR (qRT-PCR) and Western blot (WB). Results. Six differentially expressed microRNAs (DEMs) were identified, and 8 target genes were selected after screening. By using the KM Plotter software, miRNA-124 and ARHGEF3 were obviously associated with the overall survival of patients with osteosarcoma. Furthermore, ARHGEF3 was found downregulated in osteosarcoma cells by performing qRT-PCR and WB experiments. Results also showed that downregulated ARHGEF3 may associate with invasion, metastasis, and proliferation. Conclusions. By using microarray and bioinformatics analysis, DEMs were selected, and a complete miRNA-mRNA network was constructed. ARHGEF3 may act as a therapeutic and prognostic target of osteosarcoma. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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