Acute promyelocytic leukemia derived extracellular vesicles conserve PML-RARα transcript from storage-inflicted degradation: a stable diagnosis tool in APL patients.
The early death, which is more common in acute promyelocytic leukemia (APL) patients rather than other types of acute myelocytic leukemia (AML) highlights the importance of appropriate diagnostic method for early detection of this disease. The low sensitivity of the conventional methods, low tumor b...
| Publicado en: | Annals of Hematology Vol. 100; no. 9; pp. 2241 - 2253 |
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| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Sep2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=151860516&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 151860516 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09395555 1WU jtl: Annals of Hematology issn: 09395555 maglogo: N pubinfo: dt: Sep2021 vid: 100 iid: 9 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 151860516 151300390 151860516 NLM34236496 10.1007/s00277-021-04579-9 NLM34236496 151860516 ppf: 2241 ppct: 12 formats: tig: atl: Acute promyelocytic leukemia derived extracellular vesicles conserve PML-RARα transcript from storage-inflicted degradation: a stable diagnosis tool in APL patients. aug: au: Barzegar, Mohieddin Farsani, Mehdi Allahbakhshian Rafiee, Mohammad Amiri, Vahid Parkhihdeh, Sayeh Rad, Fariba Mohammadi, Mohammad Hossein affil: Laboratory Hematology and Blood Banking, School of Allied Medical Science, Shahid Beheshti University of Medical Sciences, Darband St, Qods Sq, Tehran, Iran sug: subj: Leukemia, Promyelocytic, Acute Diagnosis Proteins Neoplastic Processes Neoplastic Processes Diagnosis Aged Genotype Cell Line, Tumor Middle Age Female Leukemia, Promyelocytic, Acute Male Adult Aged: 65+ years Middle Aged: 45-64 years Adult: 19-44 years Female Male ab: The early death, which is more common in acute promyelocytic leukemia (APL) patients rather than other types of acute myelocytic leukemia (AML) highlights the importance of appropriate diagnostic method for early detection of this disease. The low sensitivity of the conventional methods, low tumor burden in some patients, and the need for bone marrow sampling are some of the diagnostic challenges on the way of proper detection of APL. Given these, we aimed to compare the efficacy of extracellular vesicles (EVs), as a diagnostic tool, with the existing methods. RT-PCR, qPCR, and flow cytometry were applied on EVs and their corresponding associated cellular component collected from 18 APL new cases, 23 patients with minimal residual disease (MRD), and NB4 cell line. RT-PCR results were positive in both cellular and vesicular components of all new cases, NB4 cells, and EVs in contrary to MRD cases. Normalized copy numbers (NCN) of PML-RARα were 5100 and 3950 for cell and EVs, respectively (p < 0.05). There was a significant difference in the NCN of PML-RARα between cells and EVs in BM samples. Investigating the effect of storage at room temperature revealed that PML-RARα level was retained near to the baseline level in EVs, but there was a significant reduction in its copy number in the cellular component during 7 days. Taken together, given to the acceptable stability, EVs could be introduced as a non-invasive liquid biopsy that alongside existing methods could remarkably change the paradigm of APL diagnostic approaches. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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