Therapeutic potential of human neural precursor cells in diabetic retinopathy—preclinical model...Coimbra Health School Annual Meeting, June 17-19, 2021.
Background Considering the expressive number of individuals being diagnosed with diabetes worldwide, it is relevant to find medicines and treatments, in order to achieve diabetes complications, as diabetic retinopathy (DR) long-awaited regression and/or cure. The study aimed to evaluate cell therapy...
| Published in: | European Journal of Public Health Vol. 31; pp. 1 - 2 |
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| Main Authors: | , , , , , , , , , , , , , , , |
| Format: | abstract proceedings research Journal Article |
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Oxford University Press / USA
2021 Supplement
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=151875903&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 151875903 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11011262 BHW jtl: European Journal of Public Health issn: 11011262 maglogo: N pubinfo: dt: 2021 Supplement vid: 31 pid: 622 pub: Oxford University Press / USA artinfo: ui: 151875903 151875903 151875903 10.1093/eurpub/ckab120.078 151875903 ppf: 1 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Therapeutic potential of human neural precursor cells in diabetic retinopathy—preclinical model...Coimbra Health School Annual Meeting, June 17-19, 2021. aug: au: Saçaki, Claudia Sayuri Mogharbel, Bassam Felipe Stricker, Priscila Elias Ferreira Dziedzic, Dilcele Silva Moreira Irioda, Ana Carolina Perussolo, Maiara Carolina Ferreira, Fabiano Montiani Moreno, Juan Dornbusch, Peterson Sato, Mário Shiokawa, Naoye Noronha, Lucia de Galdino, Marianna Bacelar Abdelwahid, Eltyeb Franco, Célia Regina Cavichiolo Carvalho, Katherine Athayde Teixeira de affil: Pequeno Príncipe Faculties sug: subj: Stem Cells Metabolism Diabetic Retinopathy Therapy Cell Therapy Evaluation Congresses and Conferences ab: Background Considering the expressive number of individuals being diagnosed with diabetes worldwide, it is relevant to find medicines and treatments, in order to achieve diabetes complications, as diabetic retinopathy (DR) long-awaited regression and/or cure. The study aimed to evaluate cell therapy with human neural precursor cells (hNPCs) on induced diabetic retinopathy (DR) in Wistar rats. Methods Wharton's Jelly Mesenchymal stem cells (WJ-MSCs) were isolated, expanded, and seeded onto a biopolymer substrate without growth factors to develop neurospheres, then hNPCs were obtained and characterized by immunocytochemistry. The animals were divided into three groups; non-diabetic (ND) n = 4; diabetic without treatment (DM) n = 9; diabetic with cell therapy (DM + hNPCs) n = 9. Cells were transplanted by intravitreal injection (1 x 106 cel/ µ L) into each of Streptozotocin (STZ) induced diabetes rats. Evaluations by Optical Coherence Tomography (OCT) and Electroretinography (ERG) were done before and after diabetes induction and post cell therapy. Four weeks after treatment, eye enucleation allowed histopathological and immunohistochemistry (IHC) analysis. Results hNPCs increased the number of retina ganglion cells, ameliorated the photoreceptor layer, and decreased the microvessel points, evidenced by ERG, OCT, histopathological, and IHC findings. The most relevant differences in morphological analysis (treated vs untreated), exhibit the retinal improvement in many layers, notably in the retinal pigment epithelium and photoreceptors. Conclusions hNPCs reduced DR progression, as demonstrated by a neuroprotective effect and promotion of retinal regeneration. pubtype: Academic Journal doctype: abstract proceedings research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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