Dean migration of unfocused micron sized particles in low aspect ratio spiral microchannels.
We present an analysis of the microfluidic Dean migration of 2.5 µm particles, which do not meet focus criterion, in tall and low aspect ratio microchannels. We demonstrate the use of such low aspect ratio and tall spirals (h > 50 µm) for isolating high concentration (> 106 particles or cells/mL) mi...
| Publicado en: | Biomedical Microdevices Vol. 23; no. 3; pp. 1 - 17 |
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| Autores principales: | , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Sep2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=151900297&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 151900297 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Sep2021 vid: 23 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 151900297 10.1007/s10544-021-00575-y 151900297 ppf: 1 ppct: 16 formats: fmt: @attributes: type: P tig: atl: Dean migration of unfocused micron sized particles in low aspect ratio spiral microchannels. aug: au: Duraiswamy, Suhanya Yung, Lin Yue Lanry affil: Department of Chemical Engineering, Indian Institute of Technology, 502285, Hyderabad, Telangana, India sug: ab: We present an analysis of the microfluidic Dean migration of 2.5 µm particles, which do not meet focus criterion, in tall and low aspect ratio microchannels. We demonstrate the use of such low aspect ratio and tall spirals (h > 50 µm) for isolating high concentration (> 106 particles or cells/mL) micron sized particles without an initial off-chip dilution step. We specifically show the need for a sheath fluid for isolation and systematically analyze the particle stream profile (i.e. thickness and distance from the channel wall) as a function of downstream channel length and curvature ratio, with changes in the fluid velocity and the flow rate ratio of particles to sheath fluid (FRR). We also show that the width of the particle stream can control the particle migration and that a threshold stream width and Dean drag is necessary to initiate the particle stream migration from the channel wall. We then propose a design guide based on the selection of optimum curvatures, flow velocities and the FRRs required for achieving a narrow particle stream through a particular outlet. Finally, we use the design guide to demonstrate the isolation of bacteria from bladder epithelial cells. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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