Validation of the Reference Genes for the Gene Expression Studies in Different Cell Lines of Pig.
Reverse transcription quantitative real-time polymerase chain reaction is one of the important methods to investigate gene expression in cells and tissues. However, if the data cannot be normalized with appropriate reference genes, the results may be unreliable. In this study, we detected the expres...
| Publicado en: | BioMed Research International pp. 1 - 10 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
8/19/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=152004027&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 152004027 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 8/19/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 152004027 152004027 152004027 10.1155/2021/5364190 152004027 ppf: 1 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Validation of the Reference Genes for the Gene Expression Studies in Different Cell Lines of Pig. aug: au: Xie, Chun-Di Wang, Bingyuan Shen, Zhao-Ji Yao, Wen-Ye Ao, Hong Li, Bugao Pei, Yangli Zhou, Rong affil: State Key Laboratory of Animal Nutrition sug: subj: Gene Expression Cell Line Genes Animal Studies Swine RNA Reverse Transcriptase Polymerase Chain Reaction Mutation ab: Reverse transcription quantitative real-time polymerase chain reaction is one of the important methods to investigate gene expression in cells and tissues. However, if the data cannot be normalized with appropriate reference genes, the results may be unreliable. In this study, we detected the expression of 15 reference genes in three pig cell lines. The results showed that SDHA and ALDOA were the most stable reference genes in 3D4/21 cells. TOP2B, TBP, and PPIA were the most stable reference genes in PK-15 cells. SDHA and ALDOA were the most stable reference genes in IPEC-J2 cells. In addition, each cell line only needs to use two reference genes to standardize the expression of target genes. Taken together, this study provides a reference for different pig cell lines to select reference genes and also provides a theoretical basis for the use of these cell lines in related functional researches. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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