Upregulation of Cytotoxic T-Lymphocyte- Associated Protein 4 and Forkhead Box P3 Transcripts in Peripheral Blood of Patients with Bladder Cancer.
Background: Regulatory T cells (Tregs) play a key role in the progression of tumors. These cells express forkhead box P3 (FOXP3) and cytotoxic T-lymphocyte-associated protein 4 (CTLA4), which are the potential targets for cancer immunotherapy. The present study aimed to evaluate FOXP3 and CTLA4 tran...
| Publicado en: | Iranian Journal of Medical Sciences Vol. 46; no. 5; pp. 339 - 347 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Shiraz University of Medical Sciences
Sep2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=152433314&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 152433314 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02530716 8TQH jtl: Iranian Journal of Medical Sciences issn: 02530716 maglogo: N pubinfo: dt: Sep2021 vid: 46 iid: 5 pid: 65846 pub: Shiraz University of Medical Sciences place: Shiraz, <Blank> artinfo: ui: 152433314 152433314 152433314 10.30476/ijms.2020.84462.1426 152433314 ppf: 339 ppct: 8 formats: tig: atl: Upregulation of Cytotoxic T-Lymphocyte- Associated Protein 4 and Forkhead Box P3 Transcripts in Peripheral Blood of Patients with Bladder Cancer. aug: au: Ariafar, Ali Habibagahi, Mojtaba Jaberipour, Mansooreh Khezri, Abdolaziz Hadi Khezri, Mohammad Bozorgi, Hale Hosseini, Ahmad Razmkhah, Mahboobeh affil: Department of Urology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran sug: subj: Biochemical Phenomena Bladder Neoplasms Blood T Lymphocytes Drug Effects Proteins Pharmacodynamics Killer Cells, Natural Drug Effects Human Cancer Patients Comparative Studies Genes Academic Medical Centers Iran Iran Reverse Transcriptase Polymerase Chain Reaction Quantitative Studies RNA Descriptive Statistics Data Analysis Software Gene Expression Immunotherapy ab: Background: Regulatory T cells (Tregs) play a key role in the progression of tumors. These cells express forkhead box P3 (FOXP3) and cytotoxic T-lymphocyte-associated protein 4 (CTLA4), which are the potential targets for cancer immunotherapy. The present study aimed to evaluate FOXP3 and CTLA4 transcripts in patients with bladder cancer (BC) compared with healthy individuals. Methods: Transcripts of CTLA4 and FOXP3 genes in the peripheral blood mononuclear cells (PBMCs) of 50 patients with histologically confirmed BC and 50 healthy individuals were assessed at the Institute for Cancer Research, Shiraz University of Medical Sciences (Shiraz, Iran) during 2014-2016. RNA was extracted from PBMCs, then cDNA was synthesized and subjected to quantitative real-time PCR (qRT-PCR) using appropriate primers. Statistical analysis was performed using SPSS software (version 21.0). Results: Significantly higher amounts of CTLA4 and FOXP3 gene transcripts were found in the peripheral blood of BC patients compared with healthy individuals. The expression of both genes was significantly higher in patients with non-invasive and grade I/II BC. The median of CTLA4 and FOXP3 transcript expressions was 3.74 and 5.39, respectively, in non-invasive BC patients, which was significant compared with the control group (P=0.0016 and P=0.009, respectively). The median of target gene mRNA expression in grade I/II BC patients was 2.9 for CTLA4 and 6.61 for FOXP3, which was significant compared with the controls (P=0.013 and P=0.0037, respectively). Conclusion: This study highlights the functional activity of Tregs in early stages of bladder cancer and showed the importance of CTLA4 and FOXP3, when it comes to screening BC. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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