Pediatric Thromboelastograph 6s and Laboratory Coagulation Reference Values.
* Context.--Specific reference intervals (RIs) facilitate accurate interpretation of results. Coagulation assay results may vary by demographics and also between reagents and analyzers used. Current Thromboelastograph 6s (TEG 6s) Hemostasis Analyzer RIs were generated from adult samples. Objective.-...
| Publicado en: | Archives of Pathology & Laboratory Medicine Vol. 145; no. 11; pp. 1413 - 1424 |
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| Autores principales: | , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
College of American Pathologists
Nov2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=153177834&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 153177834 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00039985 1FS jtl: Archives of Pathology & Laboratory Medicine issn: 00039985 maglogo: N pubinfo: dt: Nov2021 vid: 145 iid: 11 pid: 2550 pub: College of American Pathologists place: Northfield, Illinois artinfo: ui: 153177834 153177834 153177834 10.5858/arpa.2020-0647-OA 153177834 ppf: 1413 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Pediatric Thromboelastograph 6s and Laboratory Coagulation Reference Values. aug: au: Moynihan, Katie M. Johnson, Kerry affil: Department of Cardiology, Division of Cardiovascular Critical Care, Boston Children's Hospital, Massachusetts sug: subj: Thrombelastography In Infancy and Childhood Reference Values Blood Coagulation Human Prospective Studies Nonexperimental Studies Blood Specimen Collection Regression Partial Thromboplastin Time Prothrombin Time Infant, Newborn Infant Child, Preschool Child Adolescence Female Male Age Factors Infant, Newborn: birth-1 month Infant: 1-23 months Child, Preschool: 2-5 years Child: 6-12 years Adolescent: 13-18 years Female Male ab: * Context.--Specific reference intervals (RIs) facilitate accurate interpretation of results. Coagulation assay results may vary by demographics and also between reagents and analyzers used. Current Thromboelastograph 6s (TEG 6s) Hemostasis Analyzer RIs were generated from adult samples. Objective.--To generate reagent analyzer-specific pediatric RIs for TEG 6s and coagulation parameters. Design.--A prospective, observational, single-center study of healthy children undergoing general anesthesia (January 3, 2017 to January 3, 2019). Venous blood samples were obtained for TEG 6s (Kaolin, Kaolin-Heparinase, Rapid and Functional Fibrinogen assays) and coagulation parameters (activated partial thromboplastin time, prothrombin time, thrombin clotting time, Echis time, antithrombin activity, and fibrinogen concentration using Instrumentation Laboratory ACL-TOP analyzers). Differences between activated partial thromboplastin time and prothrombin time reagents were investigated using mixed-effects regression, comparing maximum coefficients- of-variation with assay-specific allowable variation. RIs (lower/upper limits 2.5th of 97.5th percentiles) were generated using the following 2 methods: within discrete age-groups (neonates [<1 month], infants [1 month-1 year], young children [1-5 years], older children [6-10 years], and adolescents [11-16 years]), and modeled as functions of age and/or sex using quantile regression, including significant fractional polynomial and interaction terms. Results.--Variation between prothrombin time and activated partial thromboplastin time assays using different reagents was clinically significant. Reagent-analyzer specific pediatric RIs were generated using data from 254 children. Discrete and model-based RIs varied by age for all coagulation parameters and TEG 6s variables in all assays. Conclusions.--We report reagent-analyzer specific pediatric RIs for TEG 6s and coagulation parameters. Observed variation reinforces recommendations for laboratory-specific RIs. These findings improve accuracy of interpretation of clinical results, provide a foundation for comparison and validation of tests in pathology, and illustrate feasibility and advantages of model-based RI approaches. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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