Mitochondrial oxygen monitoring with COMET: verification of calibration in man and comparison with vascular occlusion tests in healthy volunteers.
Mitochondria are the primary consumers of oxygen and therefore an important location for oxygen availability and consumption measurement. A technique has been developed for mitochondrial oxygen tension (mitoPO2) measurement, incorporated in the COMET. In contrast to most textbooks, relatively high a...
| Published in: | Journal of Clinical Monitoring & Computing Vol. 35; no. 6; pp. 1357 - 1367 |
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| Main Authors: | , , , |
| Format: | research Journal Article |
| Published: |
Springer Nature
Dec2021
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=153186665&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 153186665 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13871307 OHC jtl: Journal of Clinical Monitoring & Computing issn: 13871307 maglogo: N pubinfo: dt: Dec2021 vid: 35 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 153186665 146548011 153186665 NLM33085071 153186665 10.1007/s10877-020-00602-y NLM33085071 153186665 ppf: 1357 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Mitochondrial oxygen monitoring with COMET: verification of calibration in man and comparison with vascular occlusion tests in healthy volunteers. aug: au: Ubbink, R. Wefers Bettink, M. A. van Weteringen, W. Mik, E. G. affil: Department of Anesthesiology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands sug: subj: Porphyrins Oxygen Metabolism Mitochondria Metabolism Research Subjects Calibration Oxygen Consumption ab: Mitochondria are the primary consumers of oxygen and therefore an important location for oxygen availability and consumption measurement. A technique has been developed for mitochondrial oxygen tension (mitoPO2) measurement, incorporated in the COMET. In contrast to most textbooks, relatively high average mitoPO2 values have been reported. The first aim of this study was to verify the validity of the COMET calibration for mitoPO2 measurements in human skin. The second aim was to compare the dynamics of mitoPO2 to several other techniques assessing tissue oxygenation. Firstly, we performed a two-point calibration. Mitochondrial oxygen depletion was achieved with vascular occlusion. A high mitoPO2 was reached by local application of cyanide. MitoPO2 was compared to the arterial oxygen partial pressure (PaO2). Secondly, for deoxygenation kinetics we compared COMET variables with the LEA O2C, SenTec OxiVenT™ and Medtronic INVOS™ parameters during a vascular occlusion test. 20 healthy volunteers were recruited and resulted in 18 datasets (2 times 9 subjects). The lowest measured mitoPO2 value per subject had a median [IQR] of 3.0 [1.0-4.0] mmHg, n = 9. After cyanide application the mitoPO2 was 94.1 mmHg [87.2-110.9] and did not differ significantly (n = 9, p = 0.5) from the PaO2 of 101.0 [98.0-106.0] mmHg. In contrast to O2C, OxiVenT™ and INVOS parameters, mitoPO2 declined within seconds with pressure on the probe. The kinetics from this decline are used to mitochondrial oxygen consumption (mitoVO2). This study validates the calibration of the COMET device in humans. For mitoVO2 measurements not only blood flow cessation but application of local pressure is of great importance to clear the measurement site of oxygen-carrying erythrocytes. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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