Chrysin boosts KLF2 expression through suppression of endothelial cell-derived exosomal microRNA-92a in the model of atheroprotection.
Purpose: Atherosclerosis and its related clinical complications are the leading cause of death. MicroRNA (miR)-92a in the inflammatory endothelial dysfunction leads to atherosclerosis. Krüppel-like factor 2 (KLF2) is required for vascular integrity and endothelial function maintenance. Flavonoids po...
| Publicado en: | European Journal of Nutrition Vol. 60; no. 8; pp. 4345 - 4356 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=153436538&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 153436538 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Dec2021 vid: 60 iid: 8 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 153436538 150511035 153436538 153436538 10.1007/s00394-021-02593-1 153436538 ppf: 4345 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Chrysin boosts KLF2 expression through suppression of endothelial cell-derived exosomal microRNA-92a in the model of atheroprotection. aug: au: Lin, Chiu-Mei Wang, Bao-Wei Pan, Chun-Ming Fang, Wei-Jen Chua, Su-Kiat Cheng, Wen-Pin Shyu, Kou-Gi affil: Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan sug: subj: Flavonoids Pharmacodynamics Transcription Factors Drug Effects Gene Expression Drug Effects Endothelial Cells Drug Effects Exosomes Drug Effects MicroRNA Drug Effects Atherosclerosis Prevention and Control Carotid Arteries Drug Effects Carotid Arteries Injuries Catheters Adverse Effects Human Culture Media Blotting, Western Polymerase Chain Reaction In Situ Hybridization Immunohistochemistry Fluoroimmunoassay In Vitro Studies Descriptive Statistics Comparative Studies Antagomirs Pharmacodynamics ab: Purpose: Atherosclerosis and its related clinical complications are the leading cause of death. MicroRNA (miR)-92a in the inflammatory endothelial dysfunction leads to atherosclerosis. Krüppel-like factor 2 (KLF2) is required for vascular integrity and endothelial function maintenance. Flavonoids possess many biological properties. This study investigated the vascular protective effects of chrysin in balloon-injured carotid arteries. Materials and methods: Exosomes were extracted from human coronary artery endothelial cell (HCAEC) culture media. Herb flavonoids and chrysin were the treatments in these atheroprotective models. Western blotting and real-time PCRs were performed. In situ hybridization, immunohistochemistry, and immunofluorescence analyses were employed. Results: MiR-92a increased after balloon injury and was present in HCAEC culture media. Chrysin was treated, and significantly attenuated the miR-92a levels after balloon injury, and similar results were obtained in HCAEC cultures in vitro. Balloon injury-induced miR-92a expression, and attenuated KLF2 expression. Chrysin increased the KLF2 but reduced exosomal miR-92a secretion. The addition of chrysin and antagomir-92a, neointimal formation was reduced by 44.8 and 49.0% compared with balloon injury after 14 days, respectively. Conclusion: Chrysin upregulated KLF2 expression in atheroprotection and attenuated endothelial cell-derived miR-92a-containing exosomes. The suppressive effect of miR-92a suggests that chrysin plays an atheroprotective role. Proposed pathway for human coronary artery endothelial cell (HCAEC)-derived exosomes induced by chrysin to suppress microRNA (miR)-92a expression and counteract the inhibitory effect of miR-92a on KLF2 expression in HCAECs. This provides an outline of the critical role of the herbal flavonoid chrysin, which may serve as a valuable therapeutic supplement for atheroprotection. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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