Mediterranean diet enriched in extra-virgin olive oil or nuts modulates circulating exosomal non-coding RNAs.
Purpose: Exosomes are extracellular vesicles secreted by cells, which can transport different molecules, including nucleic acids. Dietary habits may induce gene regulation through the modulation of exosomal RNAs. We aimed at characterizing exosomal lncRNAs, mRNA and miRNAs modulation after a 1-year...
| Publicado en: | European Journal of Nutrition Vol. 60; no. 8; pp. 4279 - 4294 |
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| Autores principales: | , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=153436539&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 153436539 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Dec2021 vid: 60 iid: 8 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 153436539 150451564 153436539 153436539 10.1007/s00394-021-02594-0 153436539 ppf: 4279 ppct: 15 formats: fmt: @attributes: type: P tig: atl: Mediterranean diet enriched in extra-virgin olive oil or nuts modulates circulating exosomal non-coding RNAs. aug: au: Mantilla-Escalante, Diana C. López de las Hazas, María-Carmen Crespo, M. Carmen Martín-Hernández, Roberto Tomé-Carneiro, Joao del Pozo-Acebo, Lorena Salas-Salvadó, Jordi Bulló, Mónica Dávalos, Alberto affil: Laboratory of Epigenetics of Lipid Metabolism, Madrid Institute for Advanced Studies (IMDEA)-Food, IMDEA Food Institute, CEI UAM + CSIC, Ctra. De Cantoblanco 8, 28049, Madrid, Spain sug: subj: Mediterranean Diet Olive Oil Pharmacodynamics Nuts Pharmacodynamics RNA Drug Effects RNA, Messenger Drug Effects MicroRNA Drug Effects Diet, Fat-Restricted Human Blood Specimen Collection Exosomes Drug Effects Polymerase Chain Reaction Metabolic Networks and Pathways MicroRNA Metabolism Bioinformatics Patient Compliance ab: Purpose: Exosomes are extracellular vesicles secreted by cells, which can transport different molecules, including nucleic acids. Dietary habits may induce gene regulation through the modulation of exosomal RNAs. We aimed at characterizing exosomal lncRNAs, mRNA and miRNAs modulation after a 1-year adherence to a low-fat diet (LFD) or to Mediterranean-based diets enriched in extra-virgin olive oil (MedDiet + EVOO) or in a mixture of nuts (MedDiet + Nuts). Methods: Plasma samples were collected, at baseline and after 1 year of dietary interventions, from 150 participants included in the PREDIMED study (Reus Center). LncRNAs, mRNAs and miRNAs were isolated from plasma exosomes and screened. RT-qPCR validation was performed for miRNAs. Results: Compared with LFD, 413 lncRNAs and 188 mRNAs, and 476 lncRNAs and 235 mRNAs were differentially modulated in response to the MedDiet + EVOO and MedDiet + Nuts interventions, respectively. In addition, after 1 year of dietary interventions, 26 circulating miRNAs were identified as differentially expressed between groups. After 1 year of intervention, 11 miRNAs significantly changed in LFD group, while 8 and 21 were modulated in response to the MedDiet enriched with EVOO or nuts, respectively. Bioinformatic analyses of differentially expressed miRNAs and their validated target genes suggest certain metabolic pathways are modulated by LFD (PI3K-Akt and AMPK), MedDiet + EVOO (PI3K-Akt, NF-kappa B, HIF-1, and insulin resistance), and MedDiet-Nuts (FoxO, PI3K-Akt, AMPK, p53 and HIF-1) interventions. Conclusion: Results show that 1-year MedDiet + Nuts and MedDiet + EVOO dietary interventions modulate exosomal RNA content, with the former affecting a higher number of miRNAs. The modulation of exosomal RNAs could help explain how the adherence to a Mediterranean diet may lead to beneficial effects and deserves further investigation. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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