Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy.
A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either p...
| Publicado en: | BioMed Research International pp. 1 - 14 |
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| Autores principales: | , , , , , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
11/28/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=153829876&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 153829876 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 11/28/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 153829876 153829876 153829876 10.1155/2021/6879765 153829876 ppf: 1 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy. aug: au: Turčanová, Michaela Hrtoň, Martin Dvořák, Petr Novák, Kamil Hermanová, Markéta Bednařík, Zdeněk Polzer, Stanislav Burša, Jiří affil: Brno University of Technology, Faculty of Mechanical Engineering, Institute of Solid Mechanics, Mechatronics and Biomechanics, Technická 2896/2, Brno 616 69, Czech Republic sug: subj: Optical Imaging Methods Collagen Analysis Tissue Analysis Microscopy Methods Algorithms Animal Studies Descriptive Statistics Swine Achilles Tendon Aorta ab: A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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