Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat.
Microglial activation is a key modulator of brain vulnerability in response to intra-uterine growth restriction (IUGR). However, the consequences of IUGR on microglial development and the microglial proteome are still unknown. We used a model of IUGR induced by a gestational low-protein diet (LPD) i...
| Publicado en: | Nutrients Vol. 13; no. 11; pp. 3719 - 3720 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
MDPI
Nov2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=153874688&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 153874688 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20726643 B0TT jtl: Nutrients issn: 20726643 maglogo: N pubinfo: dt: Nov2021 vid: 13 iid: 11 pid: 97109 pub: MDPI artinfo: ui: 153874688 153874688 153874688 10.3390/nu13113719 153874688 ppf: 3719 ppct: 1 formats: tig: atl: Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat. aug: au: Zinni, Manuela Pansiot, Julien Colella, Marina Faivre, Valérie Delahaye-Duriez, Andrée Guillonneau, François Bruce, Johanna Salnot, Virginie Mairesse, Jérôme Knoop, Marit Possovre, Marie-Laure Vaiman, Daniel Baud, Olivier affil: Faculté de Médecine, Inserm UMR 1141 NeuroDiderot, Université de Paris, F-75019 Paris, France sug: subj: Fetal Growth Retardation Glial Cells Metabolism Proteomics Dietary Fats Adverse Effects Animal Studies Rats Models, Biological Gene Expression Inflammation Immune System Oxidative Stress Reactive Oxygen Species Proteins Metabolism DNA Physiology ab: Microglial activation is a key modulator of brain vulnerability in response to intra-uterine growth restriction (IUGR). However, the consequences of IUGR on microglial development and the microglial proteome are still unknown. We used a model of IUGR induced by a gestational low-protein diet (LPD) in rats. Microglia, isolated from control and growth-restricted animals at P1 and P4, showed significant changes in the proteome between the two groups. The expression of protein sets associated with fetal growth, inflammation, and the immune response were significantly enriched in LPD microglia at P1 and P4. Interestingly, upregulation of protein sets associated with the oxidative stress response and reactive oxygen species production was observed at P4 but not P1. During development, inflammation-associated proteins were upregulated between P1 and P4 in both control and LPD microglia. By contrast, proteins associated with DNA repair and senescence pathways were upregulated in only LPD microglia. Similarly, protein sets involved in protein retrograde transport were significantly downregulated in only LPD microglia. Overall, these data demonstrate significant and multiple effects of LPD-induced IUGR on the developmental program of microglial cells, leading to an abnormal proteome within the first postnatal days. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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