Recurrent Superenhancer of the Oncogene POU5F1B in Colorectal Cancers.
Superenhancer usages in single cancer form such as colorectal cancer (CRC) may provide novel efficient targeting candidates. It is unclear whether CRC contains recurrent superenhancers that confer a predisposition to malignancy. We investigated the superenhancer profile of CRC cell line HCT116 and c...
| Publicado en: | BioMed Research International pp. 1 - 12 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
12/11/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=154074840&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 154074840 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/11/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 154074840 154074840 154074840 10.1155/2021/5405060 154074840 ppf: 1 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Recurrent Superenhancer of the Oncogene POU5F1B in Colorectal Cancers. aug: au: Tao, Han-chuan Wang, Cheng Ma, Ning Zhu, Xun Zhou, Xiao-jun affil: Department of General Surgery, Dongtai Municipal People's Hospital of Nantong University, Yancheng, 224200 Jiangsu, China sug: subj: Colorectal Neoplasms Familial and Genetic Oncogenes Gene Expression Transcription Factors Analysis Cell Line, Tumor Analysis Sigmoid Analysis In Vitro Studies Human Cell Proliferation Signal Transduction Enzyme Inhibitors Pharmacodynamics Antineoplastic Agents Pharmacodynamics Gene Expression Drug Effects Polymerase Chain Reaction Oncogenes Drug Effects Cell Line, Tumor Drug Effects Descriptive Statistics ab: Superenhancer usages in single cancer form such as colorectal cancer (CRC) may provide novel efficient targeting candidates. It is unclear whether CRC contains recurrent superenhancers that confer a predisposition to malignancy. We investigated the superenhancer profile of CRC cell line HCT116 and compared it to that of a healthy sigmoid colon. We found that HCT116 had lost most of the normal colon superenhancer activities but gained a new set of tumor-favoring superenhancers that facilitate tumor proliferation, growth signalling, and hypoxia resistance. Inhibiting the superenhancers by JQ-1 treatment had significantly decreased the colony formation capability of HCT116. Then, by comparing the superenhancer genes and robust CRC upregulated genes, we identified a superenhancer associated with a common CRC upregulated oncogene, POU5f1B. POU5f1B overexpression is related to the worse outcome in CRCs. Via performing ChIP-PCR in 35 clinical samples and investigating CRC anti-H3K27ac ChiP-seq public dataset consisting of 36 samples, we further identified that the superenhancer of oncogene POU5F1B is recurrently activated in CRCs, taking 62 and 72 per cent, respectively. Moreover, JQ-1 treatment successfully inhibited the POU5F1B expression in 5 out of 6 POU5F1B superenhancer-positive samples. Therefore, we concluded that the superenhancer activation of POU5F1B contributes partially to its high expression in CRCs, in addition to the well-known gene amplification aetiology. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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