ZC3H15 Correlates with a Poor Prognosis and Tumor Progression in Melanoma.
Zinc figure CCCH-type containing 15 (ZC3H15), also called developmentally regulated GTP-binding protein 1 (DRG1) family regulatory protein 1 (DFRP1), is a zinc finger containing protein. Despite playing a role in cellular signaling, it is found overexpressed in acute myeloid leukemia and also an ind...
| Publicado en: | BioMed Research International pp. 1 - 13 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
12/27/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=154337684&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 154337684 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/27/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 154337684 154337684 154337684 10.1155/2021/8305299 154337684 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: ZC3H15 Correlates with a Poor Prognosis and Tumor Progression in Melanoma. aug: au: Li, Qian Hou, Jianbing Guo, Chengda Zhang, Yanli Liu, Lichao Hu, Huanrong Shi, Shaomin Ji, Yacong Guo, Leiyang Shi, Yaqiong Liu, Yaling Cui, Hongjuan affil: Department of Dermatology, The Third Hospital of Hebei Medical University, No. 139, Ziqiang Road, Shijiazhuang, 050051 Hebei, China sug: subj: Proteins Analysis Disease Progression Prognosis Melanoma Prognosis Signal Transduction Leukemia, Myeloid, Acute Prognosis Animal Studies Models, Biological Mice Human Genomics Immunohistochemistry Blotting, Western Polymerase Chain Reaction Cell Line, Tumor Flow Cytometry Cell Proliferation Cell Cycle Neoplasm Invasiveness Cell Migration Assays In Vitro Studies In Vivo Studies Xenografts Transferases Neoplastic Processes Proteins Metabolism ab: Zinc figure CCCH-type containing 15 (ZC3H15), also called developmentally regulated GTP-binding protein 1 (DRG1) family regulatory protein 1 (DFRP1), is a zinc finger containing protein. Despite playing a role in cellular signaling, it is found overexpressed in acute myeloid leukemia and also an independent prognostic marker in hepatocellular carcinoma patients. However, the biological effect of ZC3H15 in malignant melanoma (MM) remains unexplored. The expression of ZC3H15 in patients was analyzed using the R2: Genomics Analysis and Visualization Platform database. Immunohistochemical analysis, western blot, and qRT-PCR were used to detect ZC3H15 expression in melanoma tissues and cell lines. MTT, BrdU, flow cytometry assay, transwell, and western blot were performed to explore the proliferation, cell cycle, invasion, and migration of melanoma cells. We undertaken colony formation assay in vitro and tumor xenograft in vivo to detect the tumorigenicity of melanoma cells. In the present study, ZC3H15 was demonstrated highly expressed in melanoma tissues and cells. Elevated ZC3H15 impairs the survival of melanoma patients. Meanwhile, attenuation of ZC3H15 in melanoma cells inhibited cell proliferation and induced cycle arrest at G0/G1 phase. Consistently, the expression of cell cycle-related proteins cyclin dependent kinase 4 (CDK4), CDK6, and cyclin D1 (CCND1) was decreased while p21 was upregulated. Furthermore, we found the migration and invasion abilities were inhibited in ZC3H15-knockdown melanoma cells. In addition, downregulation of ZC3H15 resulted in inhibition of colony formation abilities in vitro and tumorigenesis in vivo. ZC3H15 promotes proliferation, migration/invasion, and tumorigenicity of melanoma cells. As a promising biomarker and therapeutic target in MM, ZC3H15 is worthy of further exploration. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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