Edible Tuber Amorphophallus paeoniifolius (Dennst.) Extract Induces Apoptosis and Suppresses Migration of Breast Cancer Cells.
Medicinal plants offer enormous possibilities in the quest of novel bioactive formulation for cancer therapy. Here, we studied the anticancer efficacy of the extract of edible tuber Amorphophallus paeoniifolius (Dennst.) (APTE) against estrogen positive MCF-7 and triple negative MDA-MB-231 breast ca...
| Publicado en: | Nutrition & Cancer Vol. 73; no. 11/12; pp. 2477 - 2491 |
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| Autores principales: | , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=154439043&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 154439043 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2021 vid: 73 iid: 11/12 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 154439043 146333572 154439043 154439043 10.1080/01635581.2020.1830127 154439043 ppf: 2477 ppct: 14 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Edible Tuber Amorphophallus paeoniifolius (Dennst.) Extract Induces Apoptosis and Suppresses Migration of Breast Cancer Cells. aug: au: Majumder, Munmi Sharma, Manoj Maiti, Siddhartha Mukhopadhyay, Rupak affil: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India sug: subj: Breast Neoplasms Drug Therapy Hormone Receptor Positive Breast Neoplasms Drug Therapy Plant Extracts Pharmacodynamics Plants, Edible Pharmacodynamics Antineoplastic Agents Pharmacodynamics Cell Line, Tumor Drug Effects Apoptosis Drug Effects Cell Movement Drug Effects In Vitro Studies Human Neoplasm Metastasis Cell Migration Inhibition Drug Effects Cell Proliferation Drug Effects Wound Healing Drug Effects Intracellular Signaling Peptides and Proteins Drug Effects Caspases Drug Effects Transferases Drug Effects Plant Extracts Analysis Chromatography, High Pressure Liquid Mass Spectrometry ab: Medicinal plants offer enormous possibilities in the quest of novel bioactive formulation for cancer therapy. Here, we studied the anticancer efficacy of the extract of edible tuber Amorphophallus paeoniifolius (Dennst.) (APTE) against estrogen positive MCF-7 and triple negative MDA-MB-231 breast cancer cell lines. APTE showed significant cytotoxic activity in both MCF-7 and MDA-MB-231 cells in a dose and time-dependent manner. The effect of APTE on metastatic parameters e.g., migration, adhesion, and invasion in MCF-7 and MDA-MB-231 cells were studied using wound healing, collagen adhesion, and transwell matrigel invasion assays, respectively. APTE significantly reduced migration in both the cell lines, however, its effect on the inhibition of adhesion and invasion was higher in MDA-MB-231 cells. Annexin V-Cy3 staining suggested that APTE induced apoptosis in these cells which was further validated by attenuation of antiapoptotic Bcl-2 and induction of pro-apoptotic Bax, Caspase-7 expression and cleavage of PARP. High resolution-liquid chromatography mass spectroscopy analysis with bioactive ethyl acetate and butanol fractions of APTE detected several compounds with anticancer activities. Overall, the study described the mechanism of anticancer activity of a common edible tuber A. paeoniifolius and contributes to growing list of naturally occurring chemo-preventive strategies. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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