Applications of Digital Microscopy and Densely Connected Convolutional Neural Networks for Automated Quantification of Babesia-Infected Erythrocytes.
BACKGROUND: Clinical babesiosis is diagnosed, and parasite burden is determined, by microscopic inspection of a thick or thin Giemsa-stained peripheral blood smear. However, quantitative analysis by manual microscopy is subject to error. As such, methods for the automated measurement of percent para...
| Publicado en: | Clinical Chemistry Vol. 68; no. 1; pp. 218 - 230 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Jan2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=154525758&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 154525758 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00099147 10CS jtl: Clinical Chemistry issn: 00099147 maglogo: N pubinfo: dt: Jan2022 vid: 68 iid: 1 pid: 622 pub: Oxford University Press / USA artinfo: ui: 154525758 154525758 154525758 10.1093/clinchem/hvab237 154525758 ppf: 218 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Applications of Digital Microscopy and Densely Connected Convolutional Neural Networks for Automated Quantification of Babesia-Infected Erythrocytes. aug: au: Durant, Thomas J. S. Dudgeon, Sarah N. McPadden, Jacob Simpson, Anisia Price, Nathan Schulz, Wade L. Torres, Richard Olson, Eben M. affil: Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT, USA sug: subj: Babesiosis Diagnosis Diagnosis, Laboratory Methods Microscopy Methods Neural Networks (Computer) Utilization Erythrocytes Analysis Automation, Laboratory Prediction Models Evaluation Human Validity Models, Statistical Evaluation Diagnostic Reference Levels Machine Learning ab: BACKGROUND: Clinical babesiosis is diagnosed, and parasite burden is determined, by microscopic inspection of a thick or thin Giemsa-stained peripheral blood smear. However, quantitative analysis by manual microscopy is subject to error. As such, methods for the automated measurement of percent parasitemia in digital microscopic images of peripheral blood smears could improve clinical accuracy, relative to the predicate method. METHODS: Individual erythrocyte images were manually labeled as "parasite" or "normal" and were used to train a model for binary image classification. The best model was then used to calculate percent parasitemia from a clinical validation dataset, and values were compared to a clinical reference value. Lastly, model interpretability was examined using an integrated gradient to identify pixels most likely to influence classification decisions. RESULTS: The precision and recall of the model during development testing were 0.92 and 1.00, respectively. In clinical validation, the model returned increasing positive signal with increasing mean reference value. However, there were 2 highly erroneous false positive values returned by the model. Further, the model incorrectly assessed 3 cases well above the clinical threshold of 10%. The integrated gradient suggested potential sources of false positives including rouleaux formations, cell boundaries, and precipitate as deterministic factors in negative erythrocyte images. CONCLUSIONS: While the model demonstrated highly accurate single cell classification and correctly assessed most slides, several false positives were highly incorrect. This project highlights the need for integrated testing of machine learning-based models, even when models in the development phase perform well. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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