Neutrophil Extracellular Traps Facilitate A549 Cell Invasion and Migration in a Macrophage-Maintained Inflammatory Microenvironment.
Introduction. The biological functions of neutrophil extracellular traps (NETs) in tumorigenesis have drawn an increasing amount of attention. This study explored the relationship between NETs and the inflammatory microenvironment in lung cancer cell invasion and metastasis. Methods. NETs were quant...
| Publicado en: | BioMed Research International pp. 1 - 12 |
|---|---|
| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
1/6/2022
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=154541242&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 154541242 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 1/6/2022 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 154541242 154541242 154541242 10.1155/2022/8316525 154541242 ppf: 1 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Neutrophil Extracellular Traps Facilitate A549 Cell Invasion and Migration in a Macrophage-Maintained Inflammatory Microenvironment. aug: au: Zhang, Lemeng Yi, Huifang Chen, Jianhua Li, Haitao Luo, Yongzhong Cheng, Tianli Yang, Hua Jiang, Zhou Pan, Changqie affil: Thoracic Medicine Department 1, Hunan Cancer Hospital, Changsha, Hunan Province 410013, China sug: subj: Lung Neoplasms Physiopathology Neutrophils Metabolism Extracellular Space Metabolism Macrophages Inflammation Risk Factors Cell Line, Tumor Neoplasm Invasiveness Risk Factors Neoplasm Metastasis Risk Factors Risk Assessment Human Staining and Labeling Oxidoreductases Fluorescent Antibody Technique Cytokines Analysis Enzyme-Linked Immunosorbent Assay In Vitro Studies Cell Migration Assays Comparative Studies DNA Lung Neoplasms Prognosis Interleukins Tumor Necrosis Factor Neoplasm Invasiveness Prevention and Control Neoplasm Metastasis Prevention and Control ab: Introduction. The biological functions of neutrophil extracellular traps (NETs) in tumorigenesis have drawn an increasing amount of attention. This study explored the relationship between NETs and the inflammatory microenvironment in lung cancer cell invasion and metastasis. Methods. NETs were quantified using myeloperoxidase (MPO–DNA) and immunofluorescence staining. Cytokine levels were measured using ELISA kits. THP-1 and A549 cells were used for in vitro experiments. Transwell and Matrigel assays were used to assess the invasion and migration abilities of the cells. Results. Neutrophil infiltration and NET formation were observed in the lung cancer tissues. Compared with healthy controls, the level of MPO–DNA complexes in lung cancer patients increased remarkably and was positively correlated with peripheral blood neutrophil counts, smoking status, and poor prognosis. Increased circulating NET levels were also positively correlated with the levels of inflammatory cytokines, including IL-1β, IL-6, IL-18, and TNF-α. Neutrophils isolated from patients with lung cancer are more prone to NET release. NETs can promote the invasion and migration ability of THP-1 and A549 cell in coculture systems, while pretreatment with NET inhibitors can effectively reduce NET-induced invasion and metastasis. The ability of NETs to promote invasion and metastasis is partly dependent on macrophages. Conclusion. Taken together, our study demonstrated that NETs facilitate A549 cell invasion and migration in a macrophage-maintained inflammatory microenvironment. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|