OPTIMIZING THE DILUTION PROTOCOL IN CONTINUOUS CELL CULTURES.
Due to its potential impact on the biotech industry, continuous cell cultures have attracted great interest in recent years. However, this type of culture remains expensive and, in some aspects, inefficient. They are often optimized based on the empirical experience of the personnel involved in thei...
| Publicado en: | Revista Cubana de Física Vol. 38; no. 2; pp. 66 - 77 |
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| Autores principales: | , |
| Formato: | Artículo |
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Universidad de La Habana
dic2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=154622323&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 154622323 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 02539268 UEW jtl: Revista Cubana de Física issn: 02539268 maglogo: N pubinfo: dt: dic2021 vid: 38 iid: 2 pid: 21208 pub: Universidad de La Habana artinfo: ui: 154622323 ppf: 66 ppct: 11 formats: fmt: @attributes: type: P size: 1.5MB tig: atl: OPTIMIZING THE DILUTION PROTOCOL IN CONTINUOUS CELL CULTURES. aug: au: PÉREZ-FERNÁNDEZ, B. A. MULET, R. affil: Group of Complex Systems and Statistical Physics, Physics Faculty, University of Havana, San L´azaro and L, 10400 La Habana, Cuba Department of Applied Physics, Physics Faculty, University of Havana, San Lázaro and L, 10400 La Habana, Cuba Department of Theoretical Physics, Physics Faculty, University of Havana, San Lázaro and L, 10400 La Habana, Cuba su: Cell culture Continuous cell lines Mathematical optimization Optimal control theory Metabolism Glucose analysis Blood lactate sug: subj: Cell culture Continuous cell lines Mathematical optimization Optimal control theory Metabolism Glucose analysis Blood lactate ab: Due to its potential impact on the biotech industry, continuous cell cultures have attracted great interest in recent years. However, this type of culture remains expensive and, in some aspects, inefficient. They are often optimized based on the empirical experience of the personnel involved in their development and maintenance, but this is time-consuming and leads to material and nutrients waste that could be reduced with a deeper understanding of the process. We propose an optimization method for continuous cell culture applying techniques from the theory of Optimum Control. As a proof of concept, we apply this method in a simple metabolic network that consumes glucose and secretes lactate. We predict the most appropriate behavior for the dilution rate that maximizes the cell density achieved in the steady state of the system. Los cultivos celulares continuos han alcanzado un potencial impacto en la industria biotecnológica, lo que ha despertado un gran interés en la comunidad científica en los últimos años. Sin embargo, este tipo de cultivo sigue siendo caro y, en algunos aspectos, ineficiente. Comúnmente, el proceso de producción es optimizado en base a la experiencia empírica del personal involucrado en su desarrollo y mantenimiento, lo cual requiere mucho tiempo y conduce a desperdicios de materiales y nutrientes que podrían reducirse con una comprensión más profunda del proceso. Nosotros proponemos un método de optimización para cultivo celular continuo utilizando Teoría de Control. Como prueba de concepto, aplicamos este método a una red metabólica simple que consume glucosa y secreta lactato. Predecimos el comportamiento de la tasa de dilución que maximiza la densidad celular alcanzada en el estado estacionario del sistema. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Cubana de Física is the property of Universidad de La Habana and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Cubana de Física holder: Universidad de La Habana dt: @attributes: year: 2021 holdings: @attributes: islocal: N |
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