Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level: A Review to Explore a Way Forward.

DNA barcodes are regarded as hereditary succession codes that serve as a recognition marker to address several queries relating to the identification, classification, community ecology, and evolution of certain functional traits in organisms. The mitochondrial cytochrome c oxidase 1 (CO1) gene as a...

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Publicado en:BioMed Research International pp. 1 - 20
Autores principales: Ahmed, Sarfraz, Ibrahim, Muhammad, Nantasenamat, Chanin, Nisar, Muhammad Farrukh, Malik, Aijaz Ahmad, Waheed, Rashem, Ahmed, Muhammad Z., Ojha, Suvash Chandra, Alam, Mohammad Khursheed
Formato: pictorial review tables/charts Journal Article
Publicado: Wiley-Blackwell 1/11/2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 1/11/2022
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      pub: Wiley-Blackwell
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        10.1155/2022/1846485
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        atl: Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level: A Review to Explore a Way Forward.
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          Ahmed, Sarfraz
          Ibrahim, Muhammad
          Nantasenamat, Chanin
          Nisar, Muhammad Farrukh
          Malik, Aijaz Ahmad
          Waheed, Rashem
          Ahmed, Muhammad Z.
          Ojha, Suvash Chandra
          Alam, Mohammad Khursheed
        affil: Department of Basic Sciences, University of Veterinary and Animal Sciences Lahore, Narowal Campus, 51600 Narowal, Pakistan
      sug:
        subj:
          DNA Analysis
          Bar Coding
          Sequence Analysis
          Enzymes
          Biological Markers
          Plants
          Bacteria
          Ecosystem
          Fungi
      ab: DNA barcodes are regarded as hereditary succession codes that serve as a recognition marker to address several queries relating to the identification, classification, community ecology, and evolution of certain functional traits in organisms. The mitochondrial cytochrome c oxidase 1 (CO1) gene as a DNA barcode is highly efficient for discriminating vertebrate and invertebrate animal species. Similarly, different specific markers are used for other organisms, including ribulose bisphosphate carboxylase (rbcL), maturase kinase (matK), transfer RNA-H and photosystem II D1-ApbsArabidopsis thaliana (trnH-psbA), and internal transcribed spacer (ITS) for plant species; 16S ribosomal RNA (16S rRNA), elongation factor Tu gene (Tuf gene), and chaperonin for bacterial strains; and nuclear ITS for fungal strains. Nevertheless, the taxon coverage of reference sequences is far from complete for genus or species-level identification. Applying the next-generation sequencing approach to the parallel acquisition of DNA barcode sequences could greatly expand the potential for library preparation or accurate identification in biodiversity research. Overall, this review articulates on the DNA barcoding technology as applied to different organisms, its universality, applicability, and innovative approach to handling DNA-based species identification.
      pubtype: Academic Journal
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        pictorial
        review
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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