Predicting RNA 5-Methylcytosine Sites by Using Essential Sequence Features and Distributions.

Methylation is one of the most common and considerable modifications in biological systems mediated by multiple enzymes. Recent studies have shown that methylation has been widely identified in different RNA molecules. RNA methylation modifications have various kinds, such as 5-methylcytosine (m5C)....

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Publicado en:BioMed Research International pp. 1 - 12
Autores principales: Chen, Lei, Li, ZhanDong, Zhang, ShiQi, Zhang, Yu-Hang, Huang, Tao, Cai, Yu-Dong
Formato: equations & formulas research tables/charts Journal Article
Publicado: Wiley-Blackwell 1/13/2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 1/13/2022
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2022/4035462
        154652586
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      tig:
        atl: Predicting RNA 5-Methylcytosine Sites by Using Essential Sequence Features and Distributions.
      aug:
        au:
          Chen, Lei
          Li, ZhanDong
          Zhang, ShiQi
          Zhang, Yu-Hang
          Huang, Tao
          Cai, Yu-Dong
        affil: School of Life Sciences, Shanghai University, Shanghai 200444, China
      sug:
        subj:
          RNA Methylation
          Proteins
          DNA Methylation
          Sequence Analysis
          Human
          Animal Studies
          Mice
          Machine Learning
          Algorithms
          Decision Trees
      ab: Methylation is one of the most common and considerable modifications in biological systems mediated by multiple enzymes. Recent studies have shown that methylation has been widely identified in different RNA molecules. RNA methylation modifications have various kinds, such as 5-methylcytosine (m5C). However, for individual methylation sites, their functions still remain to be elucidated. Testing of all methylation sites relies heavily on high-throughput sequencing technology, which is expensive and labor consuming. Thus, computational prediction approaches could serve as a substitute. In this study, multiple machine learning models were used to predict possible RNA m5C sites on the basis of mRNA sequences in human and mouse. Each site was represented by several features derived from k -mers of an RNA subsequence containing such site as center. The powerful max-relevance and min-redundancy (mRMR) feature selection method was employed to analyse these features. The outcome feature list was fed into incremental feature selection method, incorporating four classification algorithms, to build efficient models. Furthermore, the sites related to features used in the models were also investigated.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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