In Vitro Antibacterial Activity of Different Honey Samples against Clinical Isolates.

Background. The emergence of multi-drug-resistant organisms has created a lot of clinical problems. Hence, there is a need to find natural alternative treatment to counter the multi-drug-resistant organisms. Honey has a well-established usage as wound dressing in ancient and traditional medicine. Ob...

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Published in:BioMed Research International pp. 1 - 9
Main Author: Wadi, Mahasin Ahmed
Format: research tables/charts Journal Article
Published: Wiley-Blackwell 1/21/2022
Online Access:View this record in EBSCOhost
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      dt: 1/21/2022
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        154799830
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        10.1155/2022/1560050
        154799830
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        atl: In Vitro Antibacterial Activity of Different Honey Samples against Clinical Isolates.
      aug:
        au: Wadi, Mahasin Ahmed
        affil: Medical Microbiology, Department of Medical Surgical College of Nursing, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia
      sug:
        subj:
          Bacterial Infections Drug Therapy
          Honey Therapeutic Use
          Antiinfective Agents Pharmacodynamics
          Bacteria Drug Effects
          Bacteria Classification
          Human
          Wound Infection
          In Vitro Studies
          Flowers
          Honey Classification
          Honey Analysis
          Hospitals, Private
          Sudan
          Microbial Culture and Sensitivity Tests Methods
          Bacteriological Techniques
          Treatment Outcomes Evaluation
      ab: Background. The emergence of multi-drug-resistant organisms has created a lot of clinical problems. Hence, there is a need to find natural alternative treatment to counter the multi-drug-resistant organisms. Honey has a well-established usage as wound dressing in ancient and traditional medicine. Objective. The objective of this study is to establish a baseline for the antibacterial activity of 32 global raw natural and commercial various honey samples against 8 clinical isolates. Methods. Thirty-two honey samples (raw and commercial honey) collected from different global countries with different floral origins were tested in vitro for antibacterial activity against 8 clinical isolates collected from patients, at private hospital from Sudan, using disk diffusion technique. The following 6 epsilometer tests (Etest), amoxicillin, ciprofloxacin, cefotaxime, chloramphenicol, gentamicin, and tetracycline, were used against 8 clinical isolates for Minimum Inhibitory Concentration (MIC). Results. The following 8 clinical isolates were identified by conventional bacteriological methods: Staphylococcus aureus, (S. aureus) Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), Proteus vulgaris (P. vulgaris), Salmonella Typhi (S. Typhi), Shigella sonnei (S. sonnei), and methicillin-resistant Staphylococcus aureus (MRSA). Both raw natural and commercial honey exhibited antibacterial properties against tested Gram-positive and Gram-negative bacteria. The tested organisms showed low sensitivity to antibiotic Etest. Conclusion. All of the bacterial species studied were uniformly receptive to all raw and commercial tested honey samples; in contrast, the tested organisms showed low sensitivity to antibiotics. Commercial honey has the same antibacterial activity as the raw natural unprocessed honey against tested clinical isolates. Thus, honey is a successful alternative to conventional antibiotics as has been proved against clinical isolates.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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