Immobilization of Urease onto Modified Egg Shell Membrane through Cross Linking.
Background: Immobilization is an approach in industry to improve stability and reusability of urease. The efficiency of this technique depends on the type of membrane and the method of stabilization.Methods: The PEI-modified egg shell membrane was used to immobilize urease by absorption and glutaral...
| Publicado en: | Iranian Biomedical Journal Vol. 26; no. 1; pp. 132 - 142 |
|---|---|
| Autores principales: | , , , |
| Formato: | Journal Article |
| Publicado: |
Pasteur Institute of Iran
Jan2022
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=154943712&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 154943712 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1028852X 783E jtl: Iranian Biomedical Journal issn: 1028852X maglogo: N pubinfo: dt: Jan2022 vid: 26 iid: 1 pid: 47405 pub: Pasteur Institute of Iran artinfo: ui: 154943712 154943712 NLM34837893 10.52547/ibj.26.2.132 NLM34837893 154943712 ppf: 132 ppct: 10 formats: tig: atl: Immobilization of Urease onto Modified Egg Shell Membrane through Cross Linking. aug: au: Morovvat, Fatemeh Samsam Shariat, Seyed Ziae Aldin Davoudi, Maryam Norouzian, Dariush affil: Department of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran sug: subj: Enzymes Urease Hydrogen-Ion Concentration Animals Kinetics Ways of Coping Questionnaire ab: Background: Immobilization is an approach in industry to improve stability and reusability of urease. The efficiency of this technique depends on the type of membrane and the method of stabilization.Methods: The PEI-modified egg shell membrane was used to immobilize urease by absorption and glutaraldehyde cross-linking methods. The membranes were characterized by Fourier-transform infrared spectroscopy (FTIR) and AFM, and Nessler method was applied to measure the kinetic of the immobilized enzymes. Finally, the storage stability (6 °C for 21 days) and reusability (until enzyme activity reached to zero) of the immobilized enzymes were investigated.Results: Based on FTIR, three new peaks were observed in both the absorption- (at 1389.7, 1230.8, and 1074.2 cm-1) and the cross-linking (at 1615-1690, 1392.7, 1450 cm-1) immobilized enzymes. The surface roughness of the native membrane was altered after PEI treatment and enzyme immobilization. The optimal pH of cross-linking immobilized enzymes was shifted to a more neutral pH, while it was alkaline in adsorption-immobilized and free enzymes. The reaction time decreased in all immobilized enzymes (100 min for free enzyme vs. 60 and 30 min after immobilizing by adsorption and cross-linking methods, respectively). The optimal temperature for all enzymes was 70 °C and they had a higher Km and a lower Vmax than free enzyme. The stability and reusability of urease were improved by both methods.Conclusion: Our findings propose these approaches as promising ways to enhance the urease efficiency for its applications in industries and medicines. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|