Phylogenicity and Virulence Profiles of Clinical Escherichia coli Isolates in the Ho Teaching Hospital of Ghana.

Background. Escherichia coli bacteria are Gram-negative, non-spore-forming aerobes or facultative anaerobic rods. Some strains are pathogenic in men while others are commensals in the gut. The pathogenic strains cause a wide array of diseases by virtue of virulence factors. The commensal strains are...

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Publicado en:BioMed Research International pp. 1 - 9
Autores principales: Deku, John Gameli, Duedu, Kwabena Obeng, Kinanyok, Silas, Kpene, Godsway Edem, Feglo, Patrick Kwame
Formato: research tables/charts Journal Article
Publicado: Wiley-Blackwell 3/2/2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 3/2/2022
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2022/1347033
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        atl: Phylogenicity and Virulence Profiles of Clinical Escherichia coli Isolates in the Ho Teaching Hospital of Ghana.
      aug:
        au:
          Deku, John Gameli
          Duedu, Kwabena Obeng
          Kinanyok, Silas
          Kpene, Godsway Edem
          Feglo, Patrick Kwame
        affil: Department of Medical Laboratory Sciences, School of Allied Health Sciences, University of Health and Allied Sciences, Ho, Ghana
      sug:
        subj:
          Escherichia Coli Infections Microbiology
          Escherichia Coli Analysis
          Phylogenetics
          Virulence
          Sociodemographic Factors
          Academic Medical Centers Ghana
          Human
          Ghana
          Cross Sectional Studies
          Polymerase Chain Reaction
          Age Factors
          Genes
      ab: Background. Escherichia coli bacteria are Gram-negative, non-spore-forming aerobes or facultative anaerobic rods. Some strains are pathogenic in men while others are commensals in the gut. The pathogenic strains cause a wide array of diseases by virtue of virulence factors. The commensal strains are generally categorized into phylogenetic groups A and B1. The aim of this study was to determine the association between phylogeny of E. coli isolates and virulence and sociodemographic characteristics of the study subjects. Method. This study was a cross-sectional study carried out from July 2018 to June 2019. E. coli isolates obtained from different clinical specimens were subjected to polymerase chain reaction to determine their phylogenetic groupings and virulence. Results. The majority of the isolates belonged to phylogroup A 101 (74.8%), and the predominant virulent gene was fimA (88.9%). There was no significant correlation between phylogenicity and virulence, except for chuA which was found in all isolates that belonged to phylogroups clade I and D. None of the 101 isolates that belonged to group A had the chuA virulence gene. There was a significant association between patient age category and phylogenetic groups B1 and D. Conclusions. This study assessed the relationship between the phylogenetic distribution and the virulence profile of clinical isolates of E. coli. The virulence of isolates belonging to phylogroup A, which are generally considered as commensals, is alarming. Measures must therefore be put in place to control the spread of these virulent E. coli.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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