Identification of the circ_PRKDC/miR‐20a‐3p/RASA1 axis in regulating HaCaT keratinocyte migration.
Migration of keratinocytes plays a crucial role in the re‐epithelialization phase during wound healing. Circular RNA (circRNA) protein kinase, DNA‐activated, catalytic subunit (circ_PRKDC, hsa_circ_0084443) has been identified as a regulator of keratinocyte migration. However, the molecular basis go...
| Publicado en: | Wound Repair & Regeneration Vol. 30; no. 2; pp. 282 - 292 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Mar2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=155552430&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 155552430 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Mar2022 vid: 30 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 155552430 154061602 155552430 155552430 10.1111/wrr.12988 155552430 ppf: 282 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Identification of the circ_PRKDC/miR‐20a‐3p/RASA1 axis in regulating HaCaT keratinocyte migration. aug: au: Jiang, Li‐na Ji, Xiaohui Liu, Wei Qi, Chuanchuan Zhai, Xiaomei affil: Department of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou Henan,, China sug: subj: RNA, Circular Physiology MicroRNA Physiology Keratinocytes Physiology Protein Kinases Physiology DNA Physiology Cell Movement Molecular Biology Human Wound Healing Skin Physiology RNA, Circular Blood MicroRNA Blood Polymerase Chain Reaction Methods Blotting, Western Cell Migration Assays Methods Gene Expression Transcription Factors ab: Migration of keratinocytes plays a crucial role in the re‐epithelialization phase during wound healing. Circular RNA (circRNA) protein kinase, DNA‐activated, catalytic subunit (circ_PRKDC, hsa_circ_0084443) has been identified as a regulator of keratinocyte migration. However, the molecular basis governing it remains unclear. The levels of circ_PRKDC, microRNA (miR)‐20a‐3p, and RAS p21 protein activator 1 (RASA1) were assessed by quantitative real‐time PCR (qRT‐PCR) or western blot. Subcellular localization, Actinomycin D, and Ribonuclease (RNase) R assays were performed to characterise circ_PRKDC. Cell migration was gauged by transwell and wound‐healing assays. A direct relationship between miR‐20a‐3p and circ_PRKDC or RASA1 was verified by dual‐luciferase reporter and RNA pull‐down assays. Circ_PRKDC expression was reduced in wound skin during wound healing. Circ_PRKDC modulated migration of HaCaT keratinocytes. Mechanistically, circ_PRKDC directly targeted miR‐20a‐3p. The regulation of circ_PRKDC on HaCaT keratinocyte migration was mediated by miR‐20a‐3p. RASA1 was identified as a direct and functional target of miR‐20a‐3p, and miR‐20a‐3p‐mediated inhibition of RASA1 impacted HaCaT keratinocyte migration. Circ_PRKDC acted as a post‐transcriptional modulator of RASA1 expression through miR‐20a‐3p. Moreover, circ_PRKDC modulated migration of HaCaT keratinocytes by RASA1. Our findings demonstrated a novel molecular basis, the miR‐20a‐3p/RASA1 axis, for the regulation of circ_PRKDC on HaCaT keratinocyte migration. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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