Effects of advanced glycation end products on dental pulp calcification.
Objective: The main aim of this study was to elucidate the effects of advanced glycation end products (AGEs) on the calcification of cultured rat dental pulp cells (RDPCs) and to investigate the crystallisation ability of glycated collagen. Materials and Methods: AGEs were prepared via non‐enzymatic...
| Publicado en: | Oral Diseases Vol. 28; no. 3; pp. 745 - 756 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Apr2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=155658820&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 155658820 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1354523X DZP jtl: Oral Diseases issn: 1354523X maglogo: Y pubinfo: dt: Apr2022 vid: 28 iid: 3 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 155658820 148646296 155658820 155658820 10.1111/odi.13792 155658820 ppf: 745 ppct: 11 formats: tig: atl: Effects of advanced glycation end products on dental pulp calcification. aug: au: Sugiyama, Keita Miura, Jiro Shimizu, Masato Takashima, Aoi Matsuda, Yusuke Kayashima, Hiroki Okamoto, Motoki Nagashima, Tadashi Araki, Tsutomu affil: Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Osaka, Japan sug: subj: Glycation End Products, Advanced Analysis Dental Pulp Diseases Calcinosis Cell Line Animal Studies Rats In Vitro Studies Staining and Labeling Immunohistochemistry Reverse Transcriptase Polymerase Chain Reaction Microscopy, Electron, Scanning Microscopy, Electron Spectrum Analysis Lactate Dehydrogenase Alkaline Phosphatase Analysis of Variance Post Hoc Analysis Descriptive Statistics Cell Proliferation Chromatography, Liquid Cell Culture Techniques Gene Expression Genetic Markers Crystallization Diabetes Mellitus ab: Objective: The main aim of this study was to elucidate the effects of advanced glycation end products (AGEs) on the calcification of cultured rat dental pulp cells (RDPCs) and to investigate the crystallisation ability of glycated collagen. Materials and Methods: AGEs were prepared via non‐enzymatic glycation of a dish coated with type I collagen using dl‐glyceraldehyde. To investigate the effects of AGEs on RDPCs, we performed WST‐1 and lactate dehydrogenase assays; alkaline phosphatase, Alizarin Red S and immunohistochemical staining; and real‐time quantitative reverse transcription PCR. In addition, we performed crystallisation experiments on glycated collagen. All microstructures were analysed using scanning electron microscopy/energy‐dispersive X‐ray spectroscopy and transmission electron microscopy/diffraction pattern analysis. Results: AGEs did not affect the proliferation or differentiation of RDPCs, but enhanced the calcification rate and cytotoxicity. No major calcification‐related genes or proteins were involved in these calcifications, and glycated collagen was found to exhibit a negative polarity and form calcium phosphate crystals. Cytotoxicity due to drastic changes in the concentration of pericellular ions led to dystrophic calcification, assumed to represent an aspect of diabetic pulp calcifications. Conclusion: Glycated collagen‐containing AGEs provide a nurturing environment for crystallisation and have a significant effect on the early calcification of RDPCs. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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