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Changes in the intestinal expression of drug metabolism-related genes in a piglet model of parenteral nutrition.

Background: Parenteral nutrition (PN) may serve as a nutritional supportive therapy accompanied by oral medication, but the effect of PN on intestinal expression of drug metabolism-related genes remains unknown. Methods: Twelve Bama piglets receiving PN for 14 days were used as in vivo model. Change...

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Publicado en:Nutrition & Metabolism Vol. 19; no. 1; pp. 1 - 12
Autores principales: Dai, Li-Na, Zhao, Yu-Ling, Jiang, Lu, Yan, Jun-Kai
Formato: pictorial research tables/charts Journal Article
Publicado: BioMed Central 3/9/2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 3/9/2022
      vid: 19
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      pub: BioMed Central
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        10.1186/s12986-022-00654-8
        155685166
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        atl: Changes in the intestinal expression of drug metabolism-related genes in a piglet model of parenteral nutrition.
      aug:
        au:
          Dai, Li-Na
          Zhao, Yu-Ling
          Jiang, Lu
          Yan, Jun-Kai
        affil: Department of Pediatric Surgery, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, 1665 Kongjiang Rd, 200092, Shanghai, China
      sug:
        subj:
          Parenteral Nutrition
          Intestinal Mucosa Metabolism
          Drugs Metabolism
          Gene Expression
          Animal Studies
          Models, Biological
          Swine
          In Vivo Studies
          Proteomics
          Fibroblast Growth Factors Blood
          Enzyme-Linked Immunosorbent Assay
          Carrier Proteins
          Glutathione
          In Vitro Studies
      ab: Background: Parenteral nutrition (PN) may serve as a nutritional supportive therapy accompanied by oral medication, but the effect of PN on intestinal expression of drug metabolism-related genes remains unknown. Methods: Twelve Bama piglets receiving PN for 14 days were used as in vivo model. Changes in intestinal drug metabolism-related genes were examined by proteomic analysis. Serum levels of fibroblast growth factor 19 (FGF19) were determined by ELISA, and the effect of FGF19 on the expression of drug metabolism-related genes was examined using murine ileum organoids. Results: A total of 1063 differentially expressed proteins were identified in PN group. Of note, two drug transporters (Abcb1 and Abcc2) were significantly decreased in PN group, along with two glutathione-related drug-metabolizing enzymes, glutathione peroxidase (Gpx2) and glutathione S-transferase (Gsta1). Serum FGF19 levels were dramatically reduced in PN group. Treatment with recombinant FGF19 in vitro dose-dependently up-regulated the expression of Abcb1, Abcc2, Gpx2 and Gsta1 in organoids. Conclusion: Our data indicated that intestinal drug metabolism-related genes were significantly dysregulated under PN, and some of the changed genes were attributed to gut-derived FGF19.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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