Metabotypes of flavan-3-ol colonic metabolites after cranberry intake: elucidation and statistical approaches.
Purpose: Extensive inter-individual variability exists in the production of flavan-3-ol metabolites. Preliminary metabolic phenotypes (metabotypes) have been defined, but there is no consensus on the existence of metabotypes associated with the catabolism of catechins and proanthocyanidins. This stu...
| Published in: | European Journal of Nutrition Vol. 61; no. 3; pp. 1299 - 1318 |
|---|---|
| Main Authors: | , , , , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Springer Nature
Apr2022
|
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=155757619&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 155757619 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Apr2022 vid: 61 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 155757619 153457215 155757619 155757619 10.1007/s00394-021-02692-z 155757619 ppf: 1299 ppct: 19 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Metabotypes of flavan-3-ol colonic metabolites after cranberry intake: elucidation and statistical approaches. aug: au: Mena, Pedro Favari, Claudia Acharjee, Animesh Chernbumroong, Saisakul Bresciani, Letizia Curti, Claudio Brighenti, Furio Heiss, Christian Rodriguez-Mateos, Ana Del Rio, Daniele affil: Human Nutrition Unit, Department of Food & Drug, University of Parma, Via Volturno 39, 43125, Parma, Italy sug: subj: Metabolites Colon Metabolism Polyphenols Urine Fluid Intake Cranberry Juice Human Multivariate Analysis Factor Analysis Cluster Analysis Discriminant Analysis Algorithms ab: Purpose: Extensive inter-individual variability exists in the production of flavan-3-ol metabolites. Preliminary metabolic phenotypes (metabotypes) have been defined, but there is no consensus on the existence of metabotypes associated with the catabolism of catechins and proanthocyanidins. This study aims at elucidating the presence of different metabotypes in the urinary excretion of main flavan-3-ol colonic metabolites after consumption of cranberry products and at assessing the impact of the statistical technique used for metabotyping. Methods: Data on urinary concentrations of phenyl-γ-valerolactones and 3-(hydroxyphenyl)propanoic acid derivatives from two human interventions has been used. Different multivariate statistics, principal component analysis (PCA), cluster analysis, and partial least square-discriminant analysis (PLS-DA), have been considered. Results: Data pre-treatment plays a major role on resulting PCA models. Cluster analysis based on k-means and a final consensus algorithm lead to quantitative-based models, while the expectation–maximization algorithm and clustering according to principal component scores yield metabotypes characterized by quali-quantitative differences in the excretion of colonic metabolites. PLS-DA, together with univariate analyses, has served to validate the urinary metabotypes in the production of flavan-3-ol metabolites and to confirm the robustness of the methodological approach. Conclusions: This work proposes a methodological workflow for metabotype definition and highlights the importance of data pre-treatment and clustering methods on the final outcomes for a given dataset. It represents an additional step toward the understanding of the inter-individual variability in flavan-3-ol metabolism. Trial registration: The acute study was registered at clinicaltrials.gov as NCT02517775, August 7, 2015; the chronic study was registered at clinicaltrials.gov as NCT02764749, May 6, 2016. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|