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Combinatorial Effect of Temozolomide and Naringenin in Human Glioblastoma Multiforme Cell Lines.

Glioblastoma multiforme (GBM) is a grade IV, lethal, and the most common type of brain tumor. GBM can acquire resistance to temozolomide (TMZ) recommended for its treatment. Naringenin (NAG), a flavonoid generally found in grapefruit, has antioxidant, anti-proliferative, and anti-inflammatory proper...

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Published in:Nutrition & Cancer Vol. 74; no. 3; pp. 1071 - 1079
Main Authors: Gautam, Megha, Gabrani, Reema
Format: research tables/charts Journal Article
Published: Taylor & Francis Ltd 2022
Online Access:View this record in EBSCOhost
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      dt: 2022
      vid: 74
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      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        10.1080/01635581.2021.1952438
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        atl: Combinatorial Effect of Temozolomide and Naringenin in Human Glioblastoma Multiforme Cell Lines.
      aug:
        au:
          Gautam, Megha
          Gabrani, Reema
        affil: Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India
      sug:
        subj:
          Cell Line, Tumor Drug Effects
          Glioma Drug Therapy
          Drug Therapy, Combination
          Flavonoids Pharmacodynamics
          Temozolomide Pharmacodynamics
          Human
          Cell Proliferation Drug Effects
          Colorimetry Methods
          Apoptosis Drug Effects
          Fluorescent Antibody Technique
          Drug Toxicity
          Drug Synergism
          Drug Resistance
      ab: Glioblastoma multiforme (GBM) is a grade IV, lethal, and the most common type of brain tumor. GBM can acquire resistance to temozolomide (TMZ) recommended for its treatment. Naringenin (NAG), a flavonoid generally found in grapefruit, has antioxidant, anti-proliferative, and anti-inflammatory properties. It has been reported that phytochemicals can reduce resistance and improve the efficacy of a chemo-resistant drug. The combinatorial effect of TMZ and NAG on cell proliferation was evaluated using 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay, and the apoptosis in the U87MG and LN229 GBM cells were evaluated by change in fluorescence intensity. The effect of NAG and TMZ on anchorage-independent single-cell colony formation and cell migration was investigated. NAG and TMZ demonstrated enhanced cytotoxic effects on U87MG and LN229 cell lines. The combination index value being less than one indicated the synergistic action of the two drugs in restricting the growth of the cells. The NAG and TMZ together resulted in higher fluorescence intensity as compared to the alone drug. Further, the study showed a marked reduction in the migration of the cells and the formation of a single cell colony. Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1952438
      pubtype: Academic Journal
      doctype:
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        tables/charts
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      ougenre: Article
    language: English
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