Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy.
Background: Analysis of the constituents of gallstones using various spectroscopic techniques assists in identification of the pathogenesis of gallstones. In the current study, using Fourier Transform Infra-Red (FTIR) Spectroscopy, a Gallstone Standard Library (GSL) and a Gallstone Real Patients' Li...
| Publicado en: | BMC Gastroenterology Vol. 22; no. 1; pp. 146 - 147 |
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| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
3/28/2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=156041150&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 156041150 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1471230X 1CHO jtl: BMC Gastroenterology issn: 1471230X maglogo: N pubinfo: dt: 3/28/2022 vid: 22 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 156041150 156041150 NLM35346074 156041150 10.1186/s12876-022-02227-8 NLM35346074 156041150 ppf: 146 ppct: 1 formats: tig: atl: Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy. aug: au: Jafri, Lena Abid, Muhammad Abbas Asif, Humera Hashmi, Bilal Majid, Hafsa Ghani, Farooq Ahmed, Sibtain Siddiqui, Imran Khan, Aysha Habib affil: Section Head Chemical Pathology, Department of Pathology & Laboratory Medicine, Aga Khan University, Karachi, Pakistan sug: subj: Cholelithiasis Etiology Spectrophotometry, Infrared Bilirubin Mathematics Cholesterol Human ab: Background: Analysis of the constituents of gallstones using various spectroscopic techniques assists in identification of the pathogenesis of gallstones. In the current study, using Fourier Transform Infra-Red (FTIR) Spectroscopy, a Gallstone Standard Library (GSL) and a Gallstone Real Patients' Library (GRPL) were developed and validated for gallstone composition analysis.Methods: The study was conducted at the Department of Pathology & Laboratory Medicine, Aga Khan University, Pakistan. Pure standards (cholesterol, calcium carbonate, bilirubin and bile salts) and gallstone specimens were analyzed using FTIR Nicolet iS-5 Spectrometer from Thermo Fisher Scientific, USA. Thermo Scientific™ QCheck™ algorithm, embedded within the OMNIC™ software, was used to identify the unique spectral fingerprint of the patient samples to match with known, standard material. Matching of > 75% was considered acceptable. Validation for accuracy of the library was performed for twenty analyzed gallstones at an international reference lab.Results: Concerted search analysis was performed against the developed GSL consisting of 71 "pure component" spectrum divided into 5 types to generate the library. For the Gallstone Real Patient Library (GRPL), 117 patient samples were analyzed. Ninety-eight gall stones (83.8%) out of 117 stones matched with the developed GSL. Majority stones were mixed stones (95.92%), with cholesterol being the primary component (91.83%). Results of the developed library were 100% in agreement with the reports received from the external reference lab.Conclusions: The library developed displayed good consistency and can be used for detection of gallstone composition in Pakistan and replace the traditional labor- and time-intensive chemical method of gallstone analysis. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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