Osteoblastic Cell Responses of Copper Nanoparticle Coatings on Ti-6Al-7Nb Alloy Using Electrophoretic Deposition Method.

Aim. To investigate and compare the cell cytotoxicity, proliferation, cell attachment, and morphology of human fetal osteoblasts (hFOB) cells of coated samples (titanium nanocopper (Ti Cu), titanium nanohydroxyapatite (Ti HA) and titanium nanocopper ion doped hydroxyapatite (Ti Cu/HA) and uncoated s...

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Publicado en:BioMed Research International pp. 1 - 12
Autores principales: Hameed, Hanan Ali, Hasan, Haider Ali, Luddin, Norhayati, Husein, Adam, Ariffin, Azirrawani, Alam, Mohammad Khursheed
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 4/19/2022
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: BioMed Research International
      issn: 23146133
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      dt: 4/19/2022
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        156393652
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        156393652
        10.1155/2022/3675703
        156393652
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        atl: Osteoblastic Cell Responses of Copper Nanoparticle Coatings on Ti-6Al-7Nb Alloy Using Electrophoretic Deposition Method.
      aug:
        au:
          Hameed, Hanan Ali
          Hasan, Haider Ali
          Luddin, Norhayati
          Husein, Adam
          Ariffin, Azirrawani
          Alam, Mohammad Khursheed
        affil: Prosthdontic Department, College of Dentistry, University of Babylon, Iraq
      sug:
        subj:
          Copper Analysis
          Nanoparticles
          Alloys Analysis
          Electrophoresis Methods
          Osteoblasts Analysis
          Cell Physiology
          Cells, Cultured
          Cell Line
          Cell Viability
          Cell Migration Assays
          Cytology
      ab: Aim. To investigate and compare the cell cytotoxicity, proliferation, cell attachment, and morphology of human fetal osteoblasts (hFOB) cells of coated samples (titanium nanocopper (Ti Cu), titanium nanohydroxyapatite (Ti HA) and titanium nanocopper ion doped hydroxyapatite (Ti Cu/HA) and uncoated samples (Ti) in order to assess the suitability of these surface modifications on Ti-6Al-7Nb for dental implant application. Materials and Methods. The cytotoxicity was studied by examining the hFOB cell response by MTT assessment. The cell morphology was evaluated by inverted microscopy and observed under scanning electronic microscopy (SEM). Results. MTT assay results displayed that the Cu content on the surface of Ti-6Al-7Nb alloys did not produce any cytotoxic effect on cell viability. The cell viability rate in all samples ranges from 97% to 126%, indicating that hFOB cells grew at a high proliferation rate. However, no significant differences in cell viability were observed between Ti and Ti Cu and between Ti HA and Ti Cu/HA groups. Microscopic examination demonstrated no difference in the cell morphology of hFOB among all samples. In addition, SEM observation indicated favorable adhesion and spreading of the cells on the coated and uncoated samples. Conclusions. The surface modification of Ti-6Al-7Nb alloy with Cu, HA, and Cu/HA exhibits good cell biocompatibility, and the Cu has no influence on the cell proliferation and differentiation of hFOB.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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