Proof of concept of a multimodal intravital molecular imaging system for tumour transpathology investigation.
Background: Transpathology highlights the interpretation of the underlying physiology behind molecular imaging. However, it remains challenging due to the discrepancies between in vivo and in vitro measurements and difficulties of precise co-registration between trans-scaled images. This study aims...
| Publicado en: | European Journal of Nuclear Medicine & Molecular Imaging Vol. 49; no. 4; pp. 1157 - 1166 |
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| Autores principales: | , , , , , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Mar2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=156400554&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 156400554 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16197070 NPC jtl: European Journal of Nuclear Medicine & Molecular Imaging issn: 16197070 maglogo: N pubinfo: dt: Mar2022 vid: 49 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 156400554 152996973 10.1007/s00259-021-05574-y 156400554 ppf: 1157 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Proof of concept of a multimodal intravital molecular imaging system for tumour transpathology investigation. aug: au: Liu, Zhen Cheng, Tao Düwel, Stephan Jian, Ziying Topping, Geoffrey J. Steiger, Katja Wang, Qian Braren, Rickmer Reder, Sybille Mittelhäuser, Markus Hundshammer, Christian Feuerecker, Benedikt Huang, Sung-Cheng Schwaiger, Markus Schilling, Franz Ziegler, Sibylle I. Shi, Kuangyu affil: Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany sug: ab: Background: Transpathology highlights the interpretation of the underlying physiology behind molecular imaging. However, it remains challenging due to the discrepancies between in vivo and in vitro measurements and difficulties of precise co-registration between trans-scaled images. This study aims to develop a multimodal intravital molecular imaging (MIMI) system as a tool for in vivo tumour transpathology investigation. Methods: The proposed MIMI system integrates high-resolution positron imaging, magnetic resonance imaging (MRI) and microscopic imaging on a dorsal skin window chamber on an athymic nude rat. The window chamber frame was designed to be compatible with multimodal imaging and its fiducial markers were customized for precise physical alignment among modalities. The co-registration accuracy was evaluated based on phantoms with thin catheters. For proof of concept, tumour models of the human colorectal adenocarcinoma cell line HT-29 were imaged. The tissue within the window chamber was sectioned, fixed and haematoxylin–eosin (HE) stained for comparison with multimodal in vivo imaging. Results: The final MIMI system had a maximum field of view (FOV) of 18 mm × 18 mm. Using the fiducial markers and the tubing phantom, the co-registration errors are 0.18 ± 0.27 mm between MRI and positron imaging, 0.19 ± 0.22 mm between positron imaging and microscopic imaging and 0.15 ± 0.27 mm between MRI and microscopic imaging. A pilot test demonstrated that the MIMI system provides an integrative visualization of the tumour anatomy, vasculatures and metabolism of the in vivo tumour microenvironment, which was consistent with ex vivo pathology. Conclusions: The established multimodal intravital imaging system provided a co-registered in vivo platform for trans-scale and transparent investigation of the underlying pathology behind imaging, which has the potential to enhance the translation of molecular imaging. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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