Nucleic acid-based electrochemical biosensors for rapid clinical diagnosis: advances, challenges, and opportunities.
Clinical diagnostic tests should be quick, reliable, simple to perform, and affordable for diagnosis and treatment of diseases. In this regard, owing to their novel properties, biosensors have attracted the attention of scientists as well as end-users. They are efficient, stable, and relatively chea...
| Publicado en: | Critical Reviews in Clinical Laboratory Sciences Vol. 59; no. 3; pp. 156 - 178 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial review tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
May2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=156474912&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 156474912 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10408363 1AV jtl: Critical Reviews in Clinical Laboratory Sciences issn: 10408363 maglogo: Y pubinfo: dt: May2022 vid: 59 iid: 3 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 156474912 153879612 156474912 156474912 10.1080/10408363.2021.1997898 156474912 ppf: 156 ppct: 22 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Nucleic acid-based electrochemical biosensors for rapid clinical diagnosis: advances, challenges, and opportunities. aug: au: Hashem, Abu Hossain, M. A. Motalib Marlinda, Ab Rahman Mamun, Mohammad Al Sagadevan, Suresh Shahnavaz, Zohreh Simarani, Khanom Johan, Mohd Rafie affil: Nanotechnology and Catalysis Research Centre, Institute for Advanced Studies, University of Malaya, Kuala Lumpur, Malaysia sug: subj: Nucleic Acids Diagnostic Use Electrochemical Techniques Equipment and Supplies Biosensors Utilization Point-of-Care Testing DNA RNA Nucleic Acid Hybridization Transducers ab: Clinical diagnostic tests should be quick, reliable, simple to perform, and affordable for diagnosis and treatment of diseases. In this regard, owing to their novel properties, biosensors have attracted the attention of scientists as well as end-users. They are efficient, stable, and relatively cheap. Biosensors have broad applications in medical diagnosis, including point-of-care (POC) monitoring, forensics, and biomedical research. The electrochemical nucleic acid (NA) biosensor, the latest invention in this field, combines the sensitivity of electroanalytical methods with the inherent bioselectivity of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The NA biosensor exploits the affinity of single-stranded DNA/RNA for its complementary strand and is used to detect complementary sequences of NA based on hybridization. After the NA component in the sensor detects the analyte, a catalytic reaction or binding event that generates an electrical signal in the transducer ensues. Since 2000, much progress has been made in this field, but there are still numerous challenges. This critical review describes the advances, challenges, and prospects of NA-based electrochemical biosensors for clinical diagnosis. It includes the basic principles, classification, sensing enhancement strategies, and applications of biosensors as well as their advantages, limitations, and future prospects, and thus it should be useful to academics as well as industry in the improvement and application of EC NA biosensors. pubtype: Academic Journal doctype: pictorial review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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