Epigenetic Effects of Blackberry Extract on Human Colorectal Cancer Cells.
Fruit-derived polyphenolic compounds have been shown to exert anticancer effects via epigenetic mechanisms. In this study, we investigated the effect of blackberry extract on the expression of DNMTs (Dnmt1, Dnmt3a, and Dnmt3b) and HDACs (HDAC1-4 and SIRT1) and its influence on the cellular different...
| Publicado en: | Nutrition & Cancer Vol. 74; no. 4; pp. 1446 - 1457 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=156475259&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 156475259 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2022 vid: 74 iid: 4 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 156475259 151495297 156475259 156475259 10.1080/01635581.2021.1952454 156475259 ppf: 1446 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Epigenetic Effects of Blackberry Extract on Human Colorectal Cancer Cells. aug: au: Tatar, Mohsen Varedi, Masoumeh Naghibalhossaini, Fakhraddin affil: Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran sug: subj: Epigenomics Blackberry Therapeutic Use Plant Extracts Pharmacodynamics Colorectal Neoplasms Drug Therapy Cell Line, Tumor Drug Effects Plant Extracts Therapeutic Use Gene Expression Drug Effects Cell Differentiation Drug Effects DNA Methylation Drug Effects Oncogenes Drug Effects Human Plant Extracts Administration and Dosage Epithelial Cells Drug Effects Tumor Markers, Biological Drug Effects Antigens, Tumor Drug Effects Alkaline Phosphatase Drug Effects Carrier Proteins Drug Effects Nuclear Proteins Drug Effects Transferases Drug Effects Hydrolases Drug Effects ab: Fruit-derived polyphenolic compounds have been shown to exert anticancer effects via epigenetic mechanisms. In this study, we investigated the effect of blackberry extract on the expression of DNMTs (Dnmt1, Dnmt3a, and Dnmt3b) and HDACs (HDAC1-4 and SIRT1) and its influence on the cellular differentiation and promoter DNA methylation of tumor-related genes using a panel of six human CRC cell lines. Treatment with IC20 and IC50 concentrations of blackberry extract for 72 h significantly reduced Dnmt1 and Dnmt3b transcript levels in HCT116, SW480, HT29/219, SW742, and LS180 cells in a dose-dependent manner. Blackberry also induced promoter DNA demethylation of SFRP2 and p16 genes in four tested CRC cell lines. Berry treatment, however, upregulated Dnmt3a genes in SW480, SW742, and HT29/219 cell lines. A dose-dependent and cell-type-specific reduction of HDAC1, HDAC2, and HDAC4 expressions were observed in CRC-treated cells. Treatment with berry extract induced the expression of SIRT1 gene in HCT116 and HT29/219 cells and increased the expression of two colonic epithelial cell differentiation markers, carcinoembryonic antigen (CEA) and alkaline phosphatase in LS180 cells in a time-dependent manner. This study is the first to report the epigenetic effects of blackberry in cancer cells. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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