Multi-objective optimisation of polymerase chain reaction continuous flow systems.
A surrogate-enabled multi-objective optimisation methodology for a continuous flow Polymerase Chain Reaction (CFPCR) systems is presented, which enables the effect of the applied PCR protocol and the channel width in the extension zone on four practical objectives of interest, to be explored. High f...
| Published in: | Biomedical Microdevices Vol. 24; no. 2; pp. 1 - 32 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Published: |
Springer Nature
Jun2022
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=156527093&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 156527093 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Jun2022 vid: 24 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 156527093 10.1007/s10544-022-00610-6 156527093 ppf: 1 ppct: 31 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Multi-objective optimisation of polymerase chain reaction continuous flow systems. aug: au: Zagklavara, Foteini Jimack, Peter K. Kapur, Nikil Querin, Osvaldo M. Thompson, Harvey M. affil: School of Computing, University of Leeds, LS2 9JT, Leeds, United Kingdom sug: ab: A surrogate-enabled multi-objective optimisation methodology for a continuous flow Polymerase Chain Reaction (CFPCR) systems is presented, which enables the effect of the applied PCR protocol and the channel width in the extension zone on four practical objectives of interest, to be explored. High fidelity, conjugate heat transfer (CHT) simulations are combined with Machine Learning to create accurate surrogate models of DNA amplification efficiency, total residence time, total substrate volume and pressure drop throughout the design space for a practical CFPCR device with sigmoid-shape microfluidic channels. A series of single objective optimisations are carried out which demonstrate that DNA concentration, pressure drop, total residence time and total substrate volume within a single unitcell can be improved by up to ∼ 5.7%, ∼ 80.5%, ∼ 17.8% and ∼ 43.2% respectively, for the practical cases considered. The methodology is then extended to a multi-objective problem, where a scientifically-rigorous procedure is needed to allow designers to strike appropriate compromises between the competing objectives. A series of multi-objective optimisation results are presented in the form of a Pareto surface, which show for example how manufacturing and operating cost reductions from device miniaturisation and reduced power consumption can be achieved with minimal impact on DNA amplification efficiency. DNA amplification has been found to be strongly related to the residence time in the extension zone, but not related to the residence times in denaturation and annealing zones. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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