Effects of changing from a diet with saturated fat to a diet with n-6 polyunsaturated fat on the serum metabolome in relation to cardiovascular disease risk factors.
Purpose: Replacing saturated fatty acids (SFA) with polyunsaturated fatty acids (PUFA) is associated with a reduced risk of cardiovascular disease. Yet, the changes in the serum metabolome after this replacement is not well known. Therefore, the present study aims to identify the metabolites differe...
| Publicado en: | European Journal of Nutrition Vol. 61; no. 4; pp. 2079 - 2090 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts randomized controlled trial Journal Article |
| Publicado: |
Springer Nature
Jun2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=156859882&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 156859882 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Jun2022 vid: 61 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 156859882 154561828 156859882 156859882 10.1007/s00394-021-02796-6 156859882 ppf: 2079 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Effects of changing from a diet with saturated fat to a diet with n-6 polyunsaturated fat on the serum metabolome in relation to cardiovascular disease risk factors. aug: au: Pigsborg, Kristina Gürdeniz, Gözde Rangel-Huerta, Oscar Daniel Holven, Kirsten B. Dragsted, Lars Ove Ulven, Stine M. affil: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg, Denmark sug: subj: Cardiovascular Risk Factors Prevention and Control Fatty Acids, Saturated Fatty Acids, Unsaturated Therapeutic Use Fatty Acids, Unsaturated Metabolism Metabolites Evaluation Human Adult Middle Age Aged Randomized Controlled Trials Random Assignment Double-Blind Studies Cardiometabolic Risk Factors Fatty Acids Blood Chromatography, High Pressure Liquid Mass Spectrometry Discriminant Analysis ROC Curve Phosphatidylcholines Blood Carnitine Blood Bile Acids and Salts Blood Phospholipids Blood Factor Analysis Lipoproteins, LDL Cholesterol Metabolism Cholesterol Metabolism Triglycerides Metabolism Metabolomics Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years ab: Purpose: Replacing saturated fatty acids (SFA) with polyunsaturated fatty acids (PUFA) is associated with a reduced risk of cardiovascular disease. Yet, the changes in the serum metabolome after this replacement is not well known. Therefore, the present study aims to identify the metabolites differentiating diets where six energy percentage SFA is replaced with PUFA and to elucidate the association of dietary metabolites with cardiometabolic risk markers. Methods: In an 8-week, double-blind, randomized, controlled trial, 99 moderately hyper-cholesterolemic adults (25–70 years) were assigned to a control diet (C-diet) or an experimental diet (Ex-diet). Both groups received commercially available food items with different fatty acid compositions. In the Ex-diet group, products were given where SFA was replaced mostly with n-6 PUFA. Fasting serum samples were analysed by untargeted ultra-performance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS). Pre-processed data were analysed by double cross-validated Partial Least-Squares Discriminant Analysis (PLS-DA) to detect features differentiating the two diet groups. Results: PLS-DA differentiated the metabolic profiles of the Ex-diet and the C-diet groups with an area under the curve of 0.83. The Ex-diet group showed higher levels of unsaturated phosphatidylcholine plasmalogens, an unsaturated acylcarnitine, and a secondary bile acid. The C-diet group was characterized by odd-numbered phospholipids and a saturated acylcarnitine. The Principal Component analysis scores of the serum metabolic profiles characterizing the diets were significantly associated with low-density lipoprotein cholesterol, total cholesterol, and triglyceride levels but not with glycaemia. Conclusion: The serum metabolic profiles confirmed the compliance of the participants based on their diet-specific metabolome after replacing SFA with mostly n-6 PUFA. The participants' metabolic profiles in response to the change in diet were associated with cardiovascular disease risk markers. This study was registered at clinicaltrials.gov as NCT 01679496 on September 6th 2012. pubtype: Academic Journal doctype: research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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