Method for the Intraoperative Detection of IDH Mutation in Gliomas with Differential Mobility Spectrometry.
Isocitrate dehydrogenase (IDH) mutation status is an important factor for surgical decision-making: patients with IDH-mutated tumors are more likely to have a good long-term prognosis, and thus favor aggressive resection with more survival benefit to gain. Patients with IDH wild-type tumors have gen...
| Publicado en: | Current Oncology Vol. 29; no. 5; pp. 3252 - 3259 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
MDPI
May2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=157188012&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 157188012 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11980052 5EKK jtl: Current Oncology issn: 11980052 maglogo: N pubinfo: dt: May2022 vid: 29 iid: 5 pid: 97109 pub: MDPI artinfo: ui: 157188012 10.3390/curroncol29050265 157188012 ppf: 3252 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Method for the Intraoperative Detection of IDH Mutation in Gliomas with Differential Mobility Spectrometry. aug: au: Haapala, Ilkka Kondratev, Anton Roine, Antti Mäkelä, Meri Kontunen, Anton Karjalainen, Markus Laakso, Aki Koroknay-Pál, Päivi Nordfors, Kristiina Haapasalo, Hannu Oksala, Niku Vehkaoja, Antti Haapasalo, Joonas affil: Department of Neurosurgery, Tampere University Hospital, 33520 Tampere, Finland sug: ab: Isocitrate dehydrogenase (IDH) mutation status is an important factor for surgical decision-making: patients with IDH-mutated tumors are more likely to have a good long-term prognosis, and thus favor aggressive resection with more survival benefit to gain. Patients with IDH wild-type tumors have generally poorer prognosis and, therefore, conservative resection to avoid neurological deficit is favored. Current histopathological analysis with frozen sections is unable to identify IDH mutation status intraoperatively, and more advanced methods are therefore needed. We examined a novel method suitable for intraoperative IDH mutation identification that is based on the differential mobility spectrometry (DMS) analysis of the tumor. We prospectively obtained tumor samples from 22 patients, including 11 IDH-mutated and 11 IDH wild-type tumors. The tumors were cut in 88 smaller specimens that were analyzed with DMS. With a linear discriminant analysis (LDA) algorithm, the DMS was able to classify tumor samples with 86% classification accuracy, 86% sensitivity, and 85% specificity. Our results show that DMS is able to differentiate IDH-mutated and IDH wild-type tumors with good accuracy in a setting suitable for intraoperative use, which makes it a promising novel solution for neurosurgical practice. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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