Lensless light intensity model for quasi-spherical cell size measurement.

Quasi-spherical cell size measurement plays an important role in medical test. Traditional methods such as a microscope and a flow cytometer are either it depends on professionals and cannot be automated, or it is expensive and bulky, which are not suitable for point-of-care test. Lab-on-a-chip tech...

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Detalles Bibliográficos
Publicado en:Biomedical Microdevices Vol. 24; no. 2; pp. 1 - 14
Autores principales: Li, Jianwei, Dai, Li, Yu, Ningmei, Li, Zhengpeng, Li, Shuaijun
Formato: Journal Article
Publicado: Springer Nature Jun2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2022
      vid: 24
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      pub: Springer Nature
      place: New York, New York
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        157337070
        10.1007/s10544-021-00607-7
        157337070
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        atl: Lensless light intensity model for quasi-spherical cell size measurement.
      aug:
        au:
          Li, Jianwei
          Dai, Li
          Yu, Ningmei
          Li, Zhengpeng
          Li, Shuaijun
        affil: Faculty of Automation and Information Engineering, Xi’an University of Technology, 710048, Xi’an, China
      sug:
      ab: Quasi-spherical cell size measurement plays an important role in medical test. Traditional methods such as a microscope and a flow cytometer are either it depends on professionals and cannot be automated, or it is expensive and bulky, which are not suitable for point-of-care test. Lab-on-a-chip technology using the lensless imaging system gives a good solution for obtaining the quasi-spherical cell size. The diffraction effects and the low resolution are the two main problems faced by the lensless imaging system. In this paper, a lensless light intensity model for the quasi-spherical cell size measurement is given. First, the diffraction characteristics of a quasi-spherical cell edge are given. Then, a diffraction model at an arc edge is constructed based on the Fresnel diffraction at a straight edge. Using the diffraction model at an arc edge, we explained the mechanism of the formation of the quasi-spherical cell diffraction fringes. Finally, the light intensity of the first bright ring of the quasi-spherical cell diffraction pattern is used to achieve quasi-spherical cell size measurement. The required equipment and the measurement methods are extremely simple, very suitable for point-of-care test. The experimental results show that the proposed model can realize the statistical measurement of the quasi-spherical cells and the classification of the quasi-spherical cells with a difference of 1 μ m .
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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