Bacterial DNA Detection in the Blood of Healthy Subjects.
Background: The presence of microbiome in the blood samples of healthy individuals has been addressed. However, no information can be found on the healthy human blood microbiome of Iranian subjects. The current study is thus aimed to investigate the existence of bacteria or bacterial DNA in healthy...
| Publicado en: | Iranian Biomedical Journal Vol. 26; no. 3; pp. 230 - 240 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Pasteur Institute of Iran
May2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=157343298&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 157343298 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1028852X 783E jtl: Iranian Biomedical Journal issn: 1028852X maglogo: N pubinfo: dt: May2022 vid: 26 iid: 3 pid: 47405 pub: Pasteur Institute of Iran artinfo: ui: 157343298 157343298 NLM35280042 157343298 10.52547/ibj.26.3.230 NLM35280042 157343298 ppf: 230 ppct: 10 formats: tig: atl: Bacterial DNA Detection in the Blood of Healthy Subjects. aug: au: Raeisi, Javad Oloomi, Mana Zolfaghari, Mohammad Reza Siadat, Seyed Davar Zargar, Mohsen Pourramezan, Zahra Zolfaghari, MohammadReza affil: Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran sug: subj: Bacteria Iran DNA Sequence Analysis Methods RNA Research Subjects ab: Background: The presence of microbiome in the blood samples of healthy individuals has been addressed. However, no information can be found on the healthy human blood microbiome of Iranian subjects. The current study is thus aimed to investigate the existence of bacteria or bacterial DNA in healthy individuals.Methods: Blood samples of healthy subjects were incubated in BHI broth at 37 °C for 72 h. The 16S rRNA PCR and sequencing were performed to analyze bacterial isolates. The 16S rRNA PCR was directly carried out on DNA samples extracted from the blood of healthy individuals. Next generation sequencing (NGS) was conducted on blood samples with culture-positive results.Results: Fifty blood samples were tested, and six samples were positive by culture as confirmed by Gram staining and microscopy. The obtained 16S rRNA sequences of cultured bacterial isolates revealed the presence of Bacilli and Staphylococcus species by clustering in the GeneBank database (≥97% identity). The 16S rRNA gene sequencing results of one non-cultured blood specimen showed the presence of Burkholderia. NGS results illustrated the presence of Romboutsia, Lactobacillus, Streptococcus, Bacteroides, and Staphylococcus in the blood samples of positive cultures.Conclusion: The dormant blood microbiome of healthy individuals may give the idea that the steady transfer of bacteria into the blood does not necessarily lead to sepsis. However, the origins and identities of blood-associated bacterial rDNA sequences need more evaluation in the healthy population. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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