Sampling the skin surface chemistry for diagnosis and prognosis.
Skin and wound blotting are non‐invasive techniques used to sample the skin and wound surface chemistry, whereby a nitrocellulose membrane is applied to an intact or broken cutaneous surface to detect biomarkers. However, there has been no comprehensive review of the evidence for the techniques used...
| Publicado en: | Wound Repair & Regeneration Vol. 30; no. 4; pp. 509 - 526 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research systematic review tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jul2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=157690179&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 157690179 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jul2022 vid: 30 iid: 4 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 157690179 157472650 157690179 157690179 10.1111/wrr.13030 157690179 ppf: 509 ppct: 17 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Sampling the skin surface chemistry for diagnosis and prognosis. aug: au: Stanley, Guy H. M. Wang, Katie Daly, Patrick Lau, Christopher O'Brien, Aoife M. Hamill, Cheryl Fear, Mark Wood, Fiona M. affil: State Adult Burns Unit, Fiona Stanley Hospital, SMHS, Western Australia,, Australia sug: subj: Skin Specimen Handling Biological Markers Diagnostic Use Wounds and Injuries Diagnosis Wounds and Injuries Prognosis Human Systematic Review Medline Embase ab: Skin and wound blotting are non‐invasive techniques used to sample the skin and wound surface chemistry, whereby a nitrocellulose membrane is applied to an intact or broken cutaneous surface to detect biomarkers. However, there has been no comprehensive review of the evidence for the techniques used and data obtained to date. The primary aim of this study was to review the utilities of surface blotting for the diagnosis and prognosis of physiological, pre‐disease, and pathological states. The secondary aim was to summarise the procedural steps. A systematic literature search was conducted on 9 July 2021 using Medline, Embase, and Google Scholar databases. Investigators used McMaster's Critical Review Form for Quantitative Studies to assess quality, then performed a narrative synthesis reporting according to Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines. Twenty‐five studies were reviewed. Eighteen studies were of good quality, and seven were of moderate quality. These studies conducted skin and wound blotting on 176 animals and 1546 humans. Studies reported physiological and pathological states for diagnosis and prediction of conditions, including skin tears, wound healing, biofilm detection, and skin barrier function. The four steps for blotting are surface preparation, blot preparation, application and removal of blot, and analysis. This review demonstrates that blotting can determine the skin and wound surface chemistry using a versatile and reproducible technique. However, future research is needed to validate the technique and skin biomarkers identified. pubtype: Academic Journal doctype: pictorial research systematic review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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