Anticancer Activity and Molecular Mechanism of Momordica cochinchinensis Seed Extract in Chronic Myeloid Leukemia Cells.
Targeting Bcr-Abl is the key to the treatment of chronic myeloid leukemia. Despite great progress in the treatment of patients with chronic CML, advanced CML patients are still unable to obtain effective and safe drugs. Momordica cochinchinensis seed is the dried ripe seed of Momordica cochinchinens...
| Publicado en: | Nutrition & Cancer Vol. 74; no. 7; pp. 2644 - 2657 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=157771340&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 157771340 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2022 vid: 74 iid: 7 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 157771340 154124474 157771340 157771340 10.1080/01635581.2021.2014904 157771340 ppf: 2644 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Anticancer Activity and Molecular Mechanism of Momordica cochinchinensis Seed Extract in Chronic Myeloid Leukemia Cells. aug: au: Ai, Zhengdong Ma, Chong Wan, Ruiming Yin, Jingyi Li, Guiming Li, Yan Chen, Li affil: Department of Pathophysiology, Medical School, Kunming University of Science and Technology, Kunming, China sug: subj: Seeds Pharmacodynamics Plant Extracts Pharmacodynamics Antineoplastic Agents Cell Line, Tumor Drug Effects Leukemia, Myeloid, Chronic Metabolism Human Apoptosis Drug Effects Cell Proliferation Drug Effects Signal Transduction Drug Effects Xenografts In Vivo Studies Cell Viability Drug Effects Cell Cycle Drug Effects Caspases Physiology Intracellular Signaling Peptides and Proteins Drug Effects Transferases Drug Effects Transferases Metabolism Caspases Drug Effects ab: Targeting Bcr-Abl is the key to the treatment of chronic myeloid leukemia. Despite great progress in the treatment of patients with chronic CML, advanced CML patients are still unable to obtain effective and safe drugs. Momordica cochinchinensis seed is the dried ripe seed of Momordica cochinchinensis, which is a kind of fruit and consumed for dietary as well as medicinal uses. This study aimed to investigate the anticancer activity of Momordica cochinchinensis seed extract (MCSE) in CML cells. CML cells (KBM5 and KBM5-T315I) were treated with MCSE and analyzed for growth, apoptosis, and signal transduction. Nude mouse xenograft model was used to evaluate the antitumor activity of MCSE In Vivo. MCSE significantly reduced the cell viability of CML cells, triggered G0/G1 phase arrest in KBM5 cells and S phase arrest in KBM5-T315I cells. Concurrently, MCSE caused the activation of caspase-3, -8, -9, PARP and the degradation of Mcl-1, ultimately triggering endogenous and exogenous cell apoptosis. Meanwhile, MCSE downregulated Bcr-Abl levels and its downstream signaling pathways. Additionally, MCSE inhibited the growth of CML cells in nude mouse xenografts. Taken together, this study demonstrated the anticancer mechanism of MCSE, namely blocking Bcr-Abl and downregulating Mcl-1, and finally induced apoptosis of CML cells. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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