Sanguinarine, a major alkaloid from Zanthoxylum nitidum (Roxb.) DC., inhibits urease of Helicobacter pylori and jack bean: Susceptibility and mechanism.
Zanthoxylum nitidum (Roxb.) DC. (Z. nitidum) is a traditional Chinese medicine and mainly adopted to treat gastric ulcer, gastritis and stomach cancer. Sanguinarine (SNG), a natural alkaloid isolated from Z. nitidum , possesses significant anti- Helicobacter pylori and gastric protection effects. Ho...
| Publicado en: | Journal of Ethnopharmacology Vol. 295 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Sep2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=158013615&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 158013615 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: Sep2022 vid: 295 pid: 1004 pub: Elsevier B.V. artinfo: ui: 158013615 158013615 158013615 10.1016/j.jep.2022.115388 158013615 ppct: 1 formats: tig: atl: Sanguinarine, a major alkaloid from Zanthoxylum nitidum (Roxb.) DC., inhibits urease of Helicobacter pylori and jack bean: Susceptibility and mechanism. aug: au: Lu, Qiang Zhang, Zhenshan Xu, Yifei Chen, Yujia Li, Cailan affil: Department of Pharmaceutical Sciences, Zunyi Medical University, Zhuhai Campus, Zhuhai, 519041, PR China sug: subj: Alkaloids Pharmacodynamics Alkaloids Pharmacokinetics Microbial Culture and Sensitivity Tests Evaluation Urease Drug Effects Helicobacter Pylori Drug Effects Legumes Drug Effects Plants, Medicinal Pharmacodynamics Human Spectrophotometry Glutathione Pharmacodynamics Alkaloids Administration and Dosage Toxicity Tests Enzyme Inhibitors Pharmacodynamics Boric Acids Pharmacodynamics Sodium Compounds Pharmacodynamics Sulfhydryl Reagents Pharmacodynamics Alkaloids Physiology ab: Zanthoxylum nitidum (Roxb.) DC. (Z. nitidum) is a traditional Chinese medicine and mainly adopted to treat gastric ulcer, gastritis and stomach cancer. Sanguinarine (SNG), a natural alkaloid isolated from Z. nitidum , possesses significant anti- Helicobacter pylori and gastric protection effects. However, the underlying mechanism is sparsely elucidated. The present study aims to explore the inhibition effect, kinetics and potential mechanism of SNG against H. pylori urease (HPU) and jack bean urease (JBU). The improved spectrophotometric berthelot method was applied to estimate the inhibitory effect of SNG against HPU and JBU. The Lineweaver-Burk plots were adopted for investigating the inhibitory pattern in enzymatic kinetics. Sulfydryl-containing compounds and competitive active-site Ni2+ binding depressors were used for mechanism research. SNG remarkably suppressed the activities of HPU and JBU in concentration-and time-dependent mode with IC 50 of 0.48 ± 0.14 mM and 0.11 ± 0.02 mM, respectively, in comparison with urease retardant acetohydroxamic acid (0.06 ± 0.01 mM for HPU and 0.03 ± 0.00 mM for JBU, respectively). Kinetic analysis demonstrated that the inhibition of SNG against HPU and JBU were separately characterized by slow-binding, mixed-type and slow-binding, non-competitive type. Addition of sulfydryl-containing reagents (dithiothreitol, glutathione and L-cysteine) and competitive Ni2+ binding restrainers (boric acid and sodium fluoride) significantly abrogated the urease inhibitory effect of SNG, suggesting the significant role of the thiols and Ni2+ for the urease inhibition by SNG. By contrast, interaction with thiol groups possibly contributed to the repression of SNG on JBU. Furthermore, the urease suppression was proved to be partially reversible since the SNG-blocked enzyme could be partly reactivated by glutathione. SNG could observably inhibit H. pylori urease targeting the thiols and Ni2+, which indicated that SNG was a new urease suppressant with great promise. The present research also provided scientific evidence for the application of SNG and Z. nitidum treating H. pylori -associated gastrointestinal diseases. [Display omitted] pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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