Upregulated Expression of IL2RB Causes Disorder of Immune Microenvironment in Patients with Kawasaki Disease.

Aims. The clinical diagnosis of Kawasaki disease (KD) is not easy because of many atypical manifestations. This study is aimed at finding potential diagnostic markers and therapeutic targets for KD and analysing their correlation with immune cell infiltrations. Methods. First, we downloaded the KD d...

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Publicado en:BioMed Research International pp. 1 - 14
Autores principales: Liao, Yunfei, Ke, Ben, Long, Xiaoyan, Xu, Jianjun, Wu, Yongbing
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 7/25/2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 7/25/2022
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        158158189
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        10.1155/2022/2114699
        158158189
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        atl: Upregulated Expression of IL2RB Causes Disorder of Immune Microenvironment in Patients with Kawasaki Disease.
      aug:
        au:
          Liao, Yunfei
          Ke, Ben
          Long, Xiaoyan
          Xu, Jianjun
          Wu, Yongbing
        affil: Department of Cardiovascular Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China
      sug:
        subj:
          Interleukin 2
          Mucocutaneous Lymph Node Syndrome Immunology
          Mucocutaneous Lymph Node Syndrome Diagnosis
          Biological Markers
          Mucocutaneous Lymph Node Syndrome Therapy
          Gene Expression
          Cell Physiology
          Human
          Correlational Studies
          Algorithms
          Logistic Regression
          Genes
          T Lymphocytes
      ab: Aims. The clinical diagnosis of Kawasaki disease (KD) is not easy because of many atypical manifestations. This study is aimed at finding potential diagnostic markers and therapeutic targets for KD and analysing their correlation with immune cell infiltrations. Methods. First, we downloaded the KD dataset from the Gene Expression Omnibus (GEO) database and used R software to identify differentially expressed genes (DEGs) and perform functional correlation analysis. Then, CIBERSORT algorithm was used to evaluate immune cell infiltrations in samples. Coexpression analysis between DEGs and infiltrating immune cells was performed to screen the main infiltrating immune cells. Subsequently, the least absolute shrinkage and selection operator (LASSO) logistic regression analysis was used to screen the core genes related to KD. Finally, correlation analysis between the core genes and the main infiltrating immune cells was performed. Results. 327 DEGs were screened out in this study. Among them, 72 shared genes were the category of genes most likely to be disease-causing for they did not change before and after treatment. After analysis, it was found that expression level of IL2RB in KD tissues was significantly upregulated, the number of resting CD4+ memory T cells was decreased, and the decrease was significantly negatively correlated with the upregulated expression of IL2RB. Therefore, it was speculated that the upregulated expression of IL2RB disrupted Th1/Th2 cell differentiation balance, which led to a decrease of resting CD4+ memory T cells and finally caused disorder of immune microenvironment in patients with KD. Conclusions. Upregulated expression of IL2RB leads to disorder of immune microenvironment in patients with KD and eventually causes the occurrence and development of KD. Therefore, IL2RB may serve as a diagnostic marker and potential therapeutic target for KD.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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