Urine Cellular DNA Point Mutation and Methylation for Identifying Upper Tract Urinary Carcinoma.
Simple Summary: It's difficult to detect upper tract urothelial carcinoma at early stage. Invasive testing may increase risk of cancer recurrences in the bladder after radical nephroureterectomy. Thus, in the present study, we incorporated two-gene mutation and methylation biomarkers to conduct the...
| Publicado en: | Cancers Vol. 14; no. 14 |
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| Autores principales: | , , , , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
MDPI
Jul2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=158214136&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 158214136 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20726694 B74B jtl: Cancers issn: 20726694 maglogo: N pubinfo: dt: Jul2022 vid: 14 iid: 14 pid: 97109 pub: MDPI artinfo: ui: 158214136 158214136 158214136 10.3390/cancers14143537 158214136 ppct: 13 formats: tig: atl: Urine Cellular DNA Point Mutation and Methylation for Identifying Upper Tract Urinary Carcinoma. aug: au: Ouyang, Wei Luo, Lufeng Zhang, Junjie Xu, Ran Lu, Qiang Xu, Zhenzhou Liu, Jianye Li, Pei Zhang, Yaqun Zhou, Chuanchi Tang, Wei Wang, Zhenting Cao, Manman Xu, Genming Wang, Long affil: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha 410008, China sug: subj: DNA Urine Mutation Carcinoma, Transitional Cell Diagnosis Methylation Ureteroscopy Urinalysis Methods Carcinoma, Transitional Cell Urine Human Prospective Studies Hospitals Hematuria Diagnosis Polymerase Chain Reaction Random Forest Algorithms Descriptive Statistics Confidence Intervals Sensitivity and Specificity Liquid Biopsy ab: Simple Summary: It's difficult to detect upper tract urothelial carcinoma at early stage. Invasive testing may increase risk of cancer recurrences in the bladder after radical nephroureterectomy. Thus, in the present study, we incorporated two-gene mutation and methylation biomarkers to conduct the diagnostic tool of upper tract urothelial carcinoma and performed external validation to investigate the utility and stability of the optimal panel. It showed a highly specific and robust performance. It may be used as a replaceable approach for early detection of upper tract urothelial carcinoma, resulting in less extensive examinations in patients at low risk. Background: To improve the selection of patients for ureteroscopy, avoid excessive testing and reduce costs, we aimed to develop and validate a diagnostic urine assay for upper tract urinary carcinoma (UTUC). Methods: In this cohort study we recruited 402 patients from six Hunan hospitals who underwent ureteroscopy for hematuria, including 95 patients with UTUC and 307 patients with non-UTUC findings. Midstream morning urine samples were collected before ureteroscopy and surgery. DNA was extracted and qPCR was used to analyze mutations in TERT and FGFR3 and the methylation of NRN1. In the training set, the random forest algorithm was used to build an optimal panel. Lastly, the Beijing cohort (n = 76) was used to validate the panel. Results: The panel combining the methylation with mutation markers led to an AUC of 0.958 (95% CI: 0.933–0.975) with a sensitivity of 91.58% and a specificity of 94.79%. The panel presented a favorable diagnostic value for UTUC vs. other malignant tumors (AUC = 0.920) and UTUC vs. benign disease (AUC = 0.975). Furthermore, combining the panel with age revealed satisfactory results, with 93.68% sensitivity, 94.44% specificity, AUC = 0.970 and NPV = 98.6%. In the external validation process, the model showed an AUC of 0.971, a sensitivity of 95.83% and a specificity of 92.31, respectively. Conclusions: A novel diagnostic model for analyzing hematuria patients for the risk of UTUC was developed, which could lead to a reduction in the need for invasive examinations. Combining NRN1 methylation and gene mutation (FGFR3 and TERT) with age resulted in a validated accurate prediction model. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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